...Laurence Serre; Julie Delaroche; Angélique Vinit; Guy Schoehn; Eric Denarier; Anne Fourest-Lieuvin; Isabelle Arnal ABSTRACT Adenomatous polyposis coli (APC) is a scaffold protein with tumour suppressor properties. Mutations causing the loss of its C-terminal domain (APC-C), which bears cytoskeleton...

... such oncogene, Musashi-1 ( Msi-1 ), regulates translation of target mRNAs and is implicated in promoting tumorigenesis in the colon and other tissues. Msi-1 targets include the tumor suppressor adenomatous polyposis coli ( Apc ), a Wnt pathway antagonist lost in ∼80% of all colorectal cancers. Cell culture...

... DSH-2 and MIG-5 are required for positive regulation of SYS-1 (a C. elegans β-catenin), but MIG-5 has a stronger effect on the polarity of SYS-1 localization. We show that MIG-5 controls cortical APR-1 (the C. elegans APC) localization. DSH-2 and MIG-5 both regulate the localization of WRM-1 (another...

... fungi. The ortholog of MigA, Kar9, is frequently referred to as the functional ortholog of the human adenomatous-polyposis-poli (APC) protein in S. cerevisiae ( Liakopoulos et al., 2003 ; Miller and Rose, 1998 ). Although Kar9 possesses only a short amino acid sequence that is similar to APC...

...Austin T. Baldwin; Bryan T. Phillips ABSTRACT The APC tumor suppressor regulates diverse stem cell processes including gene regulation through Wnt–β-catenin signaling and chromosome stability through microtubule interactions, but how the disparate functions of APC are controlled is not well...

... of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new...

... of eukaryotic pathways. Although Giardia has many cell cycle regulatory components, its genome lacks all anaphase-promoting complex (APC) components. In the present study, we show that a single mitotic cyclin in Giardia is essential for progression into mitosis. Strikingly, Giardia cyclin B lacks the conserved...

... polyposis coli (APC), E-cadherin and von Hippel-Lindau (VHL) – in spindle orientation. We discuss how, in addition to their better-known functions, these proteins affect microtubule stability and cell polarity, and how their loss of function causes spindles to become misoriented. We also consider how other...

.... The protein might facilitate proteolytic degradation of meiotic cyclins via the APC. Our ability to mimic anaphase II arrest by a chemical inhibitor of the 26S proteasome is consistent with this idea. Alternatively, SMG7 could regulate cyclin levels by affecting the stability of cyclin mRNA. Our data also...

... of the destruction complex – APC and axin – and the activated Wnt co-receptor LRP6. β-catenin induced through the Wnt receptor complex was significantly more competent transcriptionally than overexpressed β-catenin, both in cultured cells and in early Xenopus embryos. Our data reveal a new step in the processing...

... inhibitors and clr6 HDAC mutations rescued temperature sensitive (ts) phenotypes of the mutants of the ubiquitin ligase complex anaphase-promoting complex/cyclosome (APC/C), which display metaphase arrest. This suppression coincided with facilitated complex formation of APC/C. Moreover, our mass spectrometry...

...Annette Grohmann; Kristina Tanneberger; Astrid Alzner; Jean Schneikert; Jürgen Behrens APC is a multifunctional tumor suppressor protein that negatively controls Wnt signaling, but also regulates cell adhesion and migration by interacting with the plasma membrane and the microtubule cytoskeleton...

...Koji Aoki; Makoto M. Taketo The adenomatous polyposis coli ( APC ) gene is a key tumor suppressor gene. Mutations in the gene have been found not only in most colon cancers but also in some other cancers, such as those of the liver. The APC gene product is a 312 kDa protein that has multiple...

...-mail: [email protected] ) 25 6 2007 © The Company of Biologists Limited 2007 2007 mDia1 MyoD SRF RhoA Adhesion-dependent signaling Quiescence Synchronized myoblasts β-catenin TCF APC Skeletal muscle formation occurs through a series of regulated events involving...

... inhibition of GSK-3β disturbs polarity development by leading to the formation of multiple axon-like processes in both control and knock-in neurons. This unpolarised outgrowth is accompanied by the symmetric delivery of membrane components to all neurites. Finally, the adenomatous polyposis coli (APC...

...Ki-Sook Park; Soung Hoo Jeon; Sung-Eun Kim; Young-Yil Bahk; Sheri L. Holmen; Bart O. Williams; Kwang-Chul Chung; Young-Joon Surh; Kang-Yell Choi Inactivating mutations in the adenomatous polyposis coli gene ( APC ), and activating mutations in RAS , occur in a majority of colorectal carcinomas...

...George A. Penman; Louie Leung; Inke S. Näthke Mutations resulting in the truncation of the adenomatous polyposis coli (APC) protein are common to most colonic tumours. The APC protein has emerged as a multifunctional protein that contributes to cytoskeletal organisation and is involved...

.... It captures and stabilizes microtubules through the microtubule-binding protein CLIP-170 near the cell cortex, leading to establishment of polarized cell morphology and directional cell migration. Furthermore, Rac1 and Cdc42 link the adenomatous polyposis coli (APC) protein to actin filaments through IQGAP1...

.../cyclosome (APC/C) preventing sister chromatid separation and cyclin degradation. In the present study, we show that Bub3 is an essential protein required during normal mitotic progression to prevent premature sister chromatid separation, missegreation and aneuploidy. We also found that Bub3 is required...

...Sonia Castillo-Lluva; Tatiana García-Muse; José Pérez-Martín Here, we identified a new member of the Fizzy-related family of APC activators, Cru1, which is required for virulence in the corn smut fungus Ustilago maydis . We show that Cru1 promotes the degradation of B-type cyclins in U. maydis...