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Keywords: AFM
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Journal Articles
J Cell Sci (2018) 131 (13): jcs209627.
Published: 4 July 2018
... was developed to simulate the application of forces exerted by the tipless AFM probe on the cell structure. The model is composed of three layers, including the cell membrane, cytoskeleton and nucleus, reconstructed from confocal imaging. The default mesh size and node number were set to be 46386 and 26931...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (18): 4189–4195.
Published: 15 September 2012
... respond to mechanical stimuli and assemble into large complexes remains poorly understood at the molecular level. In the past years, atomic force microscopy (AFM) has revolutionized the way in which biologists analyze cell surface proteins to molecular resolution. In this Commentary, we discuss how...
Journal Articles
J Cell Sci (2007) 120 (22): 3965–3976.
Published: 15 November 2007
... insight into the role of ALCAM during highly dynamic cellular interactions. Cells were attached to the AFM cantilever by concanavalin A (ConA)-mediated linkages essentially as described previously ( Wojcikiewicz et al., 2003 ). ConA-coated cantilevers were prepared as follows. Cantilevers were first...
Includes: Supplementary data
Journal Articles
J Cell Sci (2006) 119 (14): 2960–2967.
Published: 15 July 2006
...Theeraporn Puntheeranurak; Linda Wildling; Hermann J. Gruber; Rolf K. H. Kinne; Peter Hinterdorfer Atomic force microscopy (AFM) was used to probe topology, conformational changes and initial substratecarrier interactions of Na + -glucose co-transporter (SGLT1) in living cells on a single-molecule...
Journal Articles
J Cell Sci (2005) 118 (22): 5315–5323.
Published: 15 November 2005
...Clemens M. Franz; Daniel J. Müller Atomic force microscopy (AFM) can produce high-resolution topographic images of biological samples in physiologically relevant environments and is therefore well suited for the imaging of cellular surfaces. In this work we have investigated focal adhesion...
Journal Articles
J Cell Sci (2005) 118 (18): 4199–4206.
Published: 15 September 2005
... progenitor cells to fibronectin, an abundant extracellular matrix component during gastrulation. To measure this effect, we developed an assay to quantify the adhesion of single zebrafish primary mesendodermal progenitors using atomic-force microscopy (AFM). We observed significant differences in detachment...
Journal Articles
J Cell Sci (2003) 116 (12): 2531–2539.
Published: 15 June 2003
... myristate acetate (PMA) promotes LFA-1-dependent cell adhesion. The adhesion of PMA-stimulated cells to immobilized ICAM-1 was quantified in direct force measurements acquired by atomic force microscopy (AFM). Enhanced adhesion of PMA-stimulated cells to immobilized ICAM-1 stemmed from an increase...