... to ∼10 nm resolution in 2D samples and are typically phototoxic, limiting their use for live-cell imaging. RESOLFT uses photo-switchable fluorophores to achieve sub-diffraction 3D imaging ( Keller et al., 2007 ) but, like STED, requires non-standard hardware. Importantly in the context of imaging dynamic...

... and neuronal architecture. Recent work now highlights the possibility of studying immune cells in detail within their respective immune niches. This Review summarizes recent developments in tissue clearing methods and 3D imaging, with a focus on the localization and quantification of immune cells. We first...

... the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data support its function in nuclear morphology and meiosis, its involvement in chromatin organisation has not been studied in plants. Here, 3D imaging methods have been used to investigate nuclear morphology and chromatin...