... poorly understood. Here, we analyzed nuclear MTOCs (spindle-pole bodies, SPBs) and septal MTOCs in Aspergillus nidulans . They both contain γ-tubulin along with members of the family of γ-tubulin complex proteins (GCPs). Our data suggest that SPBs consist of γ-tubulin small complexes (γ-TuSCs...

... and/or chromosomal alignment. Consistent with this, mitotic spindles in Samp1-depleted cells contained significantly lower levels of β-tubulin and γ-tubulin, phenotypes that were rescued by overexpression of Samp1a–YFP. We found that Samp1 can bind directly to γ-tubulin and that Samp1 co-precipitated with γ-tubulin...

...Rosa Ramírez Cota; Neus Teixidó-Travesa; Artur Ezquerra; Susana Eibes; Cristina Lacasa; Joan Roig; Jens Lüders ABSTRACT Regulation of the γ-tubulin ring complex (γTuRC) through targeting and activation restricts nucleation of microtubules to microtubule-organizing centers (MTOCs), aiding...

... mutant β-catenin, which mimics mutations found in cancer, results in extra non-microtubule nucleating structures that contain a subset of centrosome proteins including γ-tubulin and centrin, but not polo-like kinase 4 (Plk4), SAS-6 or pericentrin. A transcriptionally inactive form of β-catenin also gives...

...Diane Colello; Carlos G. Reverte; Rachel Ward; Christopher W. Jones; Valentin Magidson; Alexey Khodjakov; Susan E. LaFlamme Microtubules nucleated from γ-tubulin ring complexes located at the centrosome regulate the localization of organelles, promote vesicular transport and direct cell migration...

... cylindrical microtubules and displaced appendages. These cells also express unique centrosome-related structures associated with a subset of centrosomal components, including γ-tubulin, centrin and PCM1. During hydroxyurea treatment, these abnormal structures become more abundant and undergo a change in shape...

..., a protein that localizes to the centrosome from S-phase to mitosis and to the nucleolus throughout interphase. Silencing of HCA66 expression resulted in failure of centrosome duplication and in the formation of monopolar spindles, reminiscent of the phenotype observed after γ-tubulin silencing...

... of Biologists Limited 2008 2008 RNAi Centrosome Microtubule Kinetochore γ-tubulin During mitosis, the microtubule network reorganizes into a bipolar spindle that ensures proper chromosome segregation by aligning the chromosomes along the metaphase plate before partitioning them equally...

... of the neuron-precursor-cell-expressed developmentally downregulated protein 1 (NEDD1) family of conserved proteins have recently been implicated in recruiting γ-tubulin and its associated proteins, which together make up the γ-tubulin ring complex (γTuRC), to the centrosome. Human NEDD1 and its Drosophila...

... antibody (Jackson), rinsed, and treated with 1 μg/ml RNAse A for 1 hour at 37°C, rinsed, and stained with 3 μM propidium iodide for 1 hour at room temperature. Plants double-labeled for microtubules and γ-tubulin were labeled with the above double labeling antibodies and 1:500 mouse monoclonal G9 anti-γ...

... proteins and proteins that bind along the length or stabilize the plus ends of microtubules. Recent work has improved our understanding of the molecular mechanisms of MT formation. In particular, it has shown that γ-tubulin and its associated proteins play key roles in microtubule nucleation and spindle...

...Brigitte Raynaud-Messina; Laurent Mazzolini; André Moisand; Anne-Marie Cirinesi; Michel Wright The assembly of the mitotic spindle after depletion of the major γ-tubulin isotype by RNA-mediated interference was assessed in the Drosophila S2 cell line. Depletion of γ-tubulin had no significant...

... and CDCrel-1, a substrate of parkin. The recruitment of parkin was apparently mediated through its binding to γ-tubulin, which has been shown to accumulate in the centrosome in response to misfolded proteins. Furthermore, the effect was abrogated by the microtubule-depolymerizing drug colchicine...

... The Company of Biologists Limited 2003 2003 Centriolar satellites PCM-1 Centrosome Pericentrin γ-Tubulin Centriolar satellites were initially identified by ultrathin section electron microscopy as electron-dense spherical granules ∼70-100 nm in diameter and localized around the centrosomes...

..., the concentration of tubulin subunits in cells would be far too low to allow microtubule nucleation. Microtubule-associated protein Microtubule-organizing centre Katanin MOR1 Centrosome γ-Tubulin Plant cell © The Company of Biologists Limited 2002 2002 (e-mail: geoffw@rsbs.anu.edu.au...

... for the formation of spindles in mitosis and sperm meiosis. However, this phosphatase was apparently dispensable for female meiotic divisions, which do not depend on centrosomes. In the cell depleted of pph-4.1 activity,localization of γ-tubulin and a Polo-like kinase homologue to the centrosome was severely...

...-specific antibody prevented the redistribution of γ-tubulin from the centrosome to the spindle in prometaphase CV-1 cells as assayed by immunofluorescence microscopy. Becauseγ-tubulin complexes can bind to pre-existing microtubule minus ends,these results could be explained by a model in which the broad...

.... , Jung M. K. , Oakley B. R. ( 1991 ). -Tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65 , 817 – 823 γ-tubulin sequence analysis GTP-binding conformational changes dynamic instability © 1992 by Company...