..., β1 integrin and paxillin. We examined how these interactions enable vimentin to regulate cell migration on collagen. In fibroblasts, depletion of vimentin increased talin-dependent activation of β1 integrin by more than 2-fold. Loss of vimentin was associated with reduction of β1 integrin clustering...

... that respond to flow, but the mechanism for sensing shear stress direction is poorly understood. We determined, by using in vitro flow systems and magnetic tweezers, that β1 integrin is a key sensor of force direction because it is activated by unidirectional, but not bidirectional, shearing forces. β1...

... with integrins. However, how ADAM9 modulates integrin functions is not known. We here show that ADAM9 knockdown increases β1 integrin levels through mechanisms that are independent of its protease activity. In ADAM9-silenced cells, adhesion to collagen and fibronectin is reduced, suggesting an altered function...

... progression, the relative abundance of fibronectin receptors, including integrins α5β1 and αvβ3, changes, thus altering the integrin composition of cell–matrix adhesions. Here, we show that enhanced αvβ3 expression can fully compensate for loss of α5β1 and other β1 integrins to support outside-in and inside...

..., with increased amounts of active β1 integrin. Hence, our data reveal a new role for Rac activation, promoted by Asef2, in modulating actomyosin contractility, which is important for regulating cell migration and adhesion dynamics. * Author for correspondence ([email protected]) 23 09 2013...

.... Cultured 4.1R −/− keratinocytes on fibronectin failed to form actin stress fibres and focal adhesions. Furthermore, in the absence of 4.1R, the surface expression, and consequently the activity of β1 integrin were reduced. These data enabled the identification of a functional role for protein 4.1R...

...)-complex subunits alters trafficking of Arf6 pathway cargo proteins, such as major histocompatibility complex class I (MHCI) and β1 integrin. Internalization of these cargoes from the plasma membrane was not affected in cells depleted of clathrin, but was modestly delayed in cells lacking AP-2. Furthermore...

...Marko Jović; Naava Naslavsky; Debora Rapaport; Mia Horowitz; Steve Caplan β1 integrins bind to the extracellular matrix and stimulate signaling pathways leading to crucial cellular functions, including proliferation, apoptosis, cell spreading and migration. Consequently, control of β1 integrin...

... with the results from the FACS analysis, AR induction was not detected. The PAP expression was observed in a punctate pattern of expression within the cytoplasm (consistent with the expected vesicular distribution) and CK18 was seen to stain diffusely throughout the cytoplasm. Fig. 2. Blocking β1 integrin...

...Xiaobo Meng; Keding Cheng; Oleg Krohkin; A. Paul Mould; Martin J. Humphries; Werner Ens; Kenneth Standing; John A. Wilkins Although the cell line K562 reportedly expresses a single species of β1 integrin, α5β1, surface staining with monoclonal antibodies JB1A, 12G10 and B3B11 to the β1 chain...

... at the critical stage when preadipocytes become permissive for adipogenic differentiation. Furthermore, as assessed by immunostaining, ADAM12 was transiently expressed at the cell surface concomitant with the reduced activity of β1 integrin. Co-immunoprecipitation studies indicated the formation of ADAM12/β1...

... receptors. Indeed, these effects were antagonized by blocking anti-β1-and anti-α6-integrin antibodies and directly induced by a stimulating anti-β1-integrin antibody. These results demonstrate that integrin-dependent cell to ECM adhesion reinforces E-cadherin-dependent cell-cell adhesion in Caco-2 cells...

... of these observations is strengthened by the fact that the β1 mutations display the same properties in both a fibroblast cell line (GD25) and a teratocarcinoma cell line (F9). The results indicate that changes in the phosphorylation state of S785 modulates β1 integrin function. *Author for correspondence (e...

... complex structures. Utilizing various null-mutant ES cell lines, we define roles for other molecules in this process. In β1 integrin-deficient ES cells, laminin-1 binds to the cell surface, but fails to organize into more morphologically complex structures. This result indicates that β1 integrin function...

...Emilio Hirsch; Lars Lohikangas; Donald Gullberg; Staffan Johansson; Reinhard Fässler ABSTRACT Antibody perturbation experiments suggested that migration, terminal differentiation and fusion of myoblasts are dependent on β1 integrin expression. In addition, several studies have postulated that β1...

... biological processes such as growth, differentiation, migration, and cell death. Of more than 20 known integrins, 10 contain the nearly ubiquitously expressed β1 integrin subunit. Disruption of the β1 integrin gene by homologous recombination allows us to assess the supposed functions of β1 containing...

... surface is unclear. To investigate the role of β1 integrins, the major receptor family for collagen, in this process, we overlaid polarized monolayers of MDCK II cells grown on permeable supports with type I collagen gel and correlated integrin polarity with the polarity of other apical and basolateral...

...Jonathan B. Weitzman; Annabel Chen; Martin E. Hemler ABSTRACT Various β1 integrins (VLA-2, VLA-3, VLA-4) have been suggested to bind directly to themselves or to each other, thus mediating cell-cell adhesion. Here we expressed the human α2 and α3 subunits in three different cell lines (human...