Issues

Summary: This Review details the latest research progress and technological advancements that are helping to unlock how nuclear organization underpins control of gene transcription and pre-mRNA splicing.

Summary: This Review highlights recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.

Summary: A model of the spectrin skeleton reveals mechanisms that are necessary to respond to external stresses. Moreover, it shows how global signals support skeletal structure and enhance its stability.

Summary: The interdependence of actin, microtubule and vimentin filaments leads to region-specific mechanical properties across the dorsoventral axis, with varying stiffness and elasticity affecting cytoplasmic behaviour.

Highlighted Article: The Rho GTPase TC10 regulates the local balance between microtubule stability and dynamics in axons through signaling from PAK2 to SCG10 and MAP1B via JNK on JIP1-positive endosomes, contributing to efficient axon outgrowth.

Highlighted Article: Optogenetic recruitment of talin to the plasma membrane promotes Rap1 activity by freeing Rap1–GTP from sequestration by SHANK3, enabling the Rap1–GTP–talin complex to activate integrins.

Summary: Loss of the inositol phosphatase INPP5E from mature photoreceptors leads to rapid outer segment degeneration marked by a failure to form new discs and structural abnormalities of the Golgi complex.

Highlighted Article: γ-secretase associates with retromer, a complex involved in retrograde protein trafficking, and its cargo proteins. γ-secretase facilitates trafficking of retromer cargoes from the endosome to the trans-Golgi network.

Highlighted Article: A robust, generically applicable nanobody-based affinity purification–mass spectrometry approach is described to identify the TAZ protein interactome in mammalian cells.

Summary: SuperResNET network analysis software provides structural target validation in situ in the intact cell from single-molecule localization super-resolution microscopy.