Issues
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Cover image
Cover Image
Cover: ARPC1B-deficient induced pluripotent stem cell-derived neutrophils (iNeutrophils) imaged using confocal microscopy. The cells were uniformly stimulated with 100 nM fMLP for 15 minutes before immunofluorescence staining of F-actin (green) and nuclear staining using Hoechst 33342 (blue). Note the abundant filopodia formed by ARPC1B-deficient iNeutrophils. See article by A. Peterson et al. (jcs261774).
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RESEARCH HIGHLIGHTS
CELL SCIENTISTS TO WATCH
OPINION
Harnessing artificial intelligence to reduce phototoxicity in live imaging
Summary: When conducting live-cell imaging, it is important to balance sample health with image quality. AI technology can help achieve this balance, allowing for reactive and gentle imaging.
REVIEW
Driving autophagy – the role of molecular motors
Summary: We review the role of molecular motors in autophagic flux, focusing on interactions and post-translational modifications, and we discuss how dysregulation of motor activity in autophagy contributes to disease.
SHORT REPORTS
2′-O-ribose methylation levels of ribosomal RNA distinguish different types of growth arrest in human dermal fibroblasts
Highlighted Article: RiboMeth-seq profiling of primary human dermal fibroblasts reveals growth arrest-specific patterns of rRNA 2′-O-methylation, which can distinguish and potentially influence specific growth phenotypes.
Native collagen VI delays early muscle stem cell differentiation
Summary: Collagen VI, a pivotal extracellular regulator of muscle homeostasis, counteracts myogenic differentiation and sustains stemness by modulating intracellular signals involved in myogenesis.
Neutrophil motility is regulated by both cell intrinsic and endothelial cell ARPC1B
Highlighted Article: By using the human iPSC system to study neutrophil motility, we show that the actin regulator ARPC1B in both neutrophils and endothelium drives neutrophil motility.
RESEARCH ARTICLES
DNA-PKcs-mediated transcriptional regulation of TOP2B drives chemoresistance in acute myeloid leukemia
Summary: A GCN5–DNA-PKcs–TOP2B transcriptional regulatory axis underlies anthracycline resistance in acute myeloid leukemia, identifying DNA-PKcs as a therapeutic target for such acquired resistance.
The oscillation of mitotic kinase governs cell cycle latches in mammalian cells
Summary: Combining modelling and experimentation, we propose a latching-gate mechanism for strict alternation of DNA replication and mitosis in human cells and show that, if the latches are broken by mutation, then endocycles ensue.
Microtubule polyglutamylation is an essential regulator of cytoskeletal integrity in Trypanosoma brucei
Summary: The polyglutamylase TTLL1 acts selectively on microtubule doublets of the flagellar axoneme in T. brucei and TTLL1 deletion indicates crosstalk between tubulin tyrosination/detyrosination and polyglutamylation.
Neuropsin promotes hippocampal synaptogenesis by regulating the expression and cleavage of L1CAM
Summary: Expression of neuropsin and cleavage of its substrate L1CAM correlate with synaptogenesis during mouse hippocampus development. Neuropsin also regulates L1CAM transcription and thus facilitates synaptogenesis.
Segregation of nascent GPCRs in the ER-to-Golgi transport by CCHCR1 via direct interaction
Highlighted Article: CCHCR1 differentially regulates the ER-to-Golgi transport, cell surface expression and signaling of two closely related G protein-coupled receptors and this sorting function is mediated through direct interaction.
Artificial tethering of constitutive centromere-associated network proteins induces CENP-A deposition without Knl2 in DT40 cells
Highlighted Article: Tethering of CENP-C or CENP-I induces CENP-A incorporation at a non-centromeric locus without Knl2, and further analyses suggest that CENP-C directly recruits HJURP for an artificial kinetochore formation.
FIRST PERSON
PUBLISHER'S NOTE
PREPRINT HIGHLIGHTS
Call for papers - Cilia and Flagella: from Basic Biology to Disease

We are welcoming submissions for our upcoming special issue: Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Submission deadline: 1 March 2025.
Biologists @ 100 - join us in Liverpool in March 2025

We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK. The final deadline for registration is 28 February 2025.
Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
How to investigate GPCR signalling in cells

Abigail Pearce and colleagues provide a critical overview of new, state-of-the-art approaches used to quantitatively study G protein-coupled receptor signalling in cells.
JCS-FocalPlane Training Grants

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 7 March 2025 (decision by week commencing 21 April 2025) and 6 June 2025 (decision by week commencing 28 July 2025).