Issues

Summary: Cells communicate over distances by releasing extracellular vesicles and non-vesicular nanoparticles. We review the diversity and heterogeneity of these structures, including several recently identified examples.

Highlighted Article: We show that the intramembrane protease SPP conducts topology surveillance of CYP51A1 by initiating quality control of incorrectly inserted molecules.

Summary: The mechanisms driving Cajal body formation are not well understood. We have found that SUMOylation of the Cajal body protein coilin contributes to Cajal body number and size, and influences Cajal body protein interactions.

Summary: The delineation of the Drosophila photoreceptor apical membrane into the rhabdomere and stalk is dependent on cross-regulation between two different cortical membrane protein complexes.

Summary: Rap1 regulation of Drosophila Canoe and its role in linking cell junctions to the cytoskeleton during morphogenesis involves two Ras-association (RA) domains that have very different functions

Highlighted Article: TTN5, a small ARF-like GTPase, is connected to the dynamic regulation of IRON-REGULATED TRANSPORTER 1 (IRT1) in the vesicular trafficking system through direct protein interaction and colocalization, linking with iron reductase activity.

Summary: Borg (Cdc42EP) proteins are known to promote septin-dependent stress fibers and acto-myosin contractility. We show that MDCK Borg5 instead limits contractility and motility as required for acquisition of a mature epithelial phenotype.

Highlighted Article: An image-based RNAi screen in Drosophila cells identified regulators of Imp/IGF2BP RNP granules. Among those, the EGFR downstream effector MAPK (Rolled) interacts with Imp and inhibits Imp granule assembly.

Summary: Our study reveals the molecular mechanism of CAMSAP3-mediated microtubule stabilization and unique functions within the CAMSAP family, impacting microtubule minus-end dynamics.

Highlighted Article: A multiplexed immunofluorescence technique called ‘Iterative Bleaching Extends Multiplexity’ (IBEX) was adapted to label all major cell types in the retina. This technique applied to any multicellular tissue where antibodies are available.