Issues

Summary: We examine the importance of bioimage analysis, obstacles to wider adoption of these important methods and ways to ensure that bioimage analysts receive the career support they need.

Summary: This Perspective compiles summaries of talks from the 20th anniversary meeting of the ImaBio consortium discussing the past two decades of advancements in key aspects of microscopy in the life sciences.

Summary: A proposal for actions needed to consolidate the development of an inclusive and open culture of data sharing in bioimaging and a highlight of the promising outlook of multidisciplinary scientific collaborations.

Summary: A description of diffraction-limited, proximity-based, super-resolution and electron microscopy methods for visualizing interorganelle contacts.

Summary: A discussion of the potential of using machine learning in microscopy, with a focus on data – how to acquire it for ML applications and what can be done with it.

Summary: This Review explores how recent advances in fluorescence and electron microscopy along with biosensors and optogenetic tools have updated long-standing models of cell–cell adhesion.

Summary: In this Review, we describe the basics of synchrotron-based tomographic phase-contrast X-ray imaging, discuss its applications for biologists and provide an outlook on the future of this emerging technique for biology.

Summary: We provide an introductory framework for fluorescence polarization microscopy, covering theory, implementation, practical considerations, and applications in studying cellular architecture and dynamics.

Summary: Correlative light and electron microscopy in plant cells to study the development of the endomembrane system of cereal endosperm provides new insights into cell biology and new tools for cereal seed research.

Summary: Super-resolution imaging reveals that the cell nucleolus is encapsulated by molecular complex of single-stranded DNA and histone H1.

Summary: The role of TvTSP5 and uropod-like cell membrane protrusion, which play crucial roles in the complex mechanisms of trichomoniasis infection, the most prevalent non-viral sexually transmitted disease.

Summary: Lipid droplet dynamics are essential for blood infection of the malaria parasite, Plasmodium falciparum, and might provide new targets for disease intervention.

Summary: Plant protoplast cultures, similar to animal iPSC ancestor cell cultures, exhibit rapid, multiscale chromatin changes and a decreasing entropy during dedifferentiation.

Summary: Gut Analysis Toolbox is a user-friendly image analysis tool for rapid and accurate characterization of neuronal distribution in 2D images of the gut wall, using custom-trained deep learning models.

Summary: We present a system for rapid precision targeting of organelles and macromolecular complexes for in situ structural biology. We couple this with optogenetics for redistribution of organelles to the cell periphery, thus avoiding the need for cryo-FIB milling.

Summary: Design and characterization of a novel FRET-based tension sensor that can detect myosin-associated forces in living cells.

Summary: ExoJ is a robust Fiji/ImageJ2 plugin tool that can be used to automate the identification of exocytosis from live-cell data and avoid manual analysis, which is prone to biases and is not reproducible.

Summary: VISION, an open-source software, enables high throughput and correlative analysis of cellular biophysical properties.

Summary: A simple and easy to implement interactive visualisation for EM data, inspired by digital maps, which compensates for the loss of field of view at high magnifications and improves data communication and analysis.

Summary: We present an array tomography workflow for whole-glomerulus analysis that aids evaluation of glomerular diseases and provides new insights into the ultrastructure and constituent cells of the glomerulus.

Summary: Expansion microscopy enables the visualisation of cell cycle-dependent distinct organellar organisation in the human pathogen Candida albicans, and is generalised to the study of other pathogenic yeasts.

Summary: A novel 3D optical mesoscopy approach allows the study of both GLUT4 protein expression levels and structural distribution within ultrathick sections of intact mouse hearts, in response to high fat diet-feeding.

Summary: Cell plasticity and analysis tool (cellPLATO) is a new Python-based tool for phenotypic clustering using features of cell migration and morphology; here it is applied to the study of human lymphocytes.

Summary: A flexible image analysis tool for detection and spatiotemporal analysis of exocytosis that works across imaging modalities, pH-sensitive fluorophores, and diverse vesicle and cell types.

Summary: Combining scanning electron microscopy with immunogold labeling is used to identify proteins on primary cilia in human and mouse pancreatic islets.