Issues
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Cover image
Cover Image
Cover: Scanning electron micrograph (SEM) of a human macrophage incubated with protocells containing ‘reprogramming’ cargoes. This experiment complemented in vivo macrophage reprogramming studies in zebrafish and suggest that such an approach may one day be translatable to the clinic to modulate the wound inflammatory response. See article by P. López-Cuevas et al. (jcs262202).
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RESEARCH HIGHLIGHTS
CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Intermediate filaments at a glance
Summary: An overview of the recent advancements in the field of IFs, exploring their general features, functions beyond structural support, and involvement in diseases.
REVIEW
Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants
Summary: A Review highlighting some of the mechanistic similarities and differences in CCV formation via CME from mammals, yeast and plants.
SHORT REPORT
N-terminal tags impair the ability of lamin A to provide structural support to the nucleus
Highlighted Article: N-terminal tags impair some functions of lamin A but not others, cautioning researchers who use tagged lamins to study the nucleus.
RESEARCH ARTICLES
Excess microtubule and F-actin formation mediates shortening and loss of primary cilia in response to a hyperosmotic milieu
Highlighted Article: Hyperosmotic stimuli induce reversible shortening and disassembly of primary cilia, with early diffusion of the pericentriolar material, by causing excessive formation of cytoplasmic microtubules and F-actin.
Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia
Summary: Investigation of the PHD finger proteins DevPF1 and DevPF2 indicates that they play crucial roles in genome reorganization in the ciliate Paramecium, potentially by regulating the coding and non-coding transcription during development.
Reprogramming macrophages with R848-loaded artificial protocells to modulate skin and skeletal wound healing
Highlighted Article: Phagocytic uptake of R848-loaded protocells reprogrammes human macrophages in vitro, and in zebrafish, models of skin and skeletal repair drives macrophages to be pro-inflammatory and improves healing of infected wounds.
MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice
Summary: CRISPR/Cas9-mediated disruption of testis-enriched protein MYCBPAP results in immotile spermatozoa with short tails and male infertility in mice.
Hominini-specific regulation of the cell cycle by stop codon readthrough of FEM1B
Summary: Translation of FEM1B mRNA continues beyond its stop codon to generate a C-terminally extended unstable isoform, and this process regulates the cell cycle.
TOOLS AND RESOURCES
When less is more – a fast TurboID knock-in approach for high-sensitivity endogenous interactome mapping
Summary: A fast CRISPR-pipeline to endogenously tag proteins of interest with labeling enzymes on their C- and N-terminus; this was used to map the interactome of different adaptor protein complexes using TurboID.
FIRST PERSON
Call for papers: Cell Biology of Mitochondria
We are welcoming submissions for our upcoming special issue: Cell Biology of Mitochondria. This issue will be coordinated by two Guest Editors: Ana J. Garcia-Saez (University of Cologne, Germany) and Heidi McBride (McGill University, Canada). Submission deadline: 1 October 2024.
Focal adhesion kinase signalling – tumour vulnerabilities and clinical opportunities
In this Review, David Schlaepfer and colleagues summarise 30 years of focal adhesion kinase (FAK) research with a view of the ongoing clinical testing of small-molecule FAK inhibitors. The authors touch on how FAK plays an important signal integration role and ultimately functions to guide cellular behaviour. Additionally, the authors discuss how FAK inhibition might present a powerful tool to influence the physiological response to other therapeutic approaches.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2024 applications: 7 September (decision by week commencing 8 October 2024); 22 November (decision by week commencing 16 December).
Biologists @ 100 - join us in Liverpool in March 2025
We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK.
Interview with Journal of Cell Science Editor Rob Parton
Read our interview with Rob Parton, a Cell Scientist to Watch, about his career journey leading him from the UK to the University of Queensland in Brisbane, Australia, the evolution of the membrane trafficking field and his advice for running a highly collaborative lab. As a Journal of Cell Science Editor, Rob brings to the journal his expertise in multiscale analysis of membrane function, membrane microdomains, lipid droplets and advanced microscopy techniques in cell biology.