Issues
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Cover image
Cover Image
Cover: β-actin filaments present in a field of view from a time-lapse total internal reflection fluorescence (TIRF) microscopy assay. The reaction contained 1 μM β-actin monomers, which polymerised to form actin filaments. The composite image depicts temporally colour-coded actin filaments over a period of ten frames (50 s). For reference, a perfectly static image would have all white filaments, whereas the presence of colour is correlated with filament dynamics. See article by B. K. Haarer et al. (jcs260540).
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RESEARCH HIGHLIGHTS
STICKY WICKET
OPINION
The cilium–centrosome axis in coupling cell cycle exit and cell fate
Summary: The cilium–centrosome complex regulates cell polarity and cell division, but does it help cells decide their fate? We discuss how this complex can influence cells to proliferate, enter quiescence or differentiate.
REVIEW
The role of lipid rafts in vesicle formation
Summary: Lipid rafts are involved in formation of transport, endocytic, exocytic, synaptic and extracellular vesicles, as well as enveloped viruses. Here, we discuss the proposed mechanisms for these processes.
SHORT REPORT
Molecular basis of Climp63-mediated ER lumen spacing
Summary: The structure of the ER lumen spacer Climp63 was predicted revealing the molecular basis for Climp63 self-association and ER sheet width regulation.
RESEARCH ARTICLES
Rab GTPase regulation of phagosome–lysosome fusion is bypassed in the presence of micromolar Ca2+
Highlighted Article: Macrophage immune cells phagocytose particulate matter, including pathogens, for killing, digestion and antigen presentation. We present new insights into regulation of this process by Ca2+.
Sec16 and Sed4 interdependently function as interaction and localization partners at ER exit sites
Summary: The Sec12 homolog Sed4 interacts with Sec16 and acts to localize Sec16 to ER exit sites. The ER luminal domain of Sed4 is involved in the self-interaction and localization to the highly curved ER domains.
Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming
Summary: Manipulation of the Rab7 adaptor protein RILP in macrophages alters extracellular vesicle-mediated cell–cell crosstalk and protects hepatocytes against an injury signal.
Lfc subcellular localization and activity is controlled by αv-class integrin
Highlighted Article: Fibronectin-bound αvβ3 integrin triggers the dissociation of the RhoA GEF Lfc from microtubules, leading to the activation of RhoA, formation of stress fibres and maturation of focal adhesions.
TOOLS AND RESOURCES
Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe
Summary: Conventional and improved dCas9-mediated CRISPRi methods were used for functional characterization of essential genes in Schizosaccharomyces pombe, showing that this approach is feasible.
Purification of human β- and γ-actin from budding yeast
Summary: Generation and characterization of human β- and γ-actin from budding yeast as an accessible and low-cost way to study the regulation of specific actin isoforms.
FIRST PERSON
PREPRINT HIGHLIGHTS
Interviews with Biologists @ 100 conference speakers

Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
The spatial choreography of mRNA biosynthesis

In their Review, André Ventura-Gomes and Maria Carmo-Fonseca detail the latest research progress and technological advancements that are helping to unlock how nuclear organisation underpins control of gene transcription and pre-mRNA splicing.
JCS-FocalPlane Training Grants

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction

In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.