Issues
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Cover image
Cover Image
Cover: Confocal microscopy image of wild-type Drosophila secretory salivary gland stained for F-actin (green), Swip-1 (magenta) and DAPI (blue). See article by F. Lehne and S. Bogdan (jcs260366).
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RESEARCH HIGHLIGHTS
EDITORIAL
CELL SCIENCE AT A GLANCE
Membrane tethers at a glance
Summary: A summary of how tethers play a key role in the recognition of specific cargo containers and in membrane fusion.
REVIEW
The role of SPIRE actin nucleators in cellular transport processes
Summary: Recent discoveries have shown that the WH2-domain-based actin nucleator SPIRE has a role in actomyosin-driven transport processes involved in mammalian reproduction, skin pigmentation and wound healing.
RESEARCH ARTICLES
Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway
Summary: Disturbed flow is a key factor in promoting endothelial dysfunction. Frizzled-4 mediates the pro-inflammatory and barrier-disrupting effects of disturbed flow in endothelial cells via a WNT5A- and β-catenin-dependent mechanism.
SARS-CoV-2 infection alkalinizes the ERGIC and lysosomes through the viroporin activity of the viral envelope protein
Summary: SARS-CoV2 infection neutralizes the pH of the ERGIC due to the proton channel activity of the viral envelope protein, which is prevented by mutations at Val25.
The dynamin-related protein Vps1 and the peroxisomal membrane protein Pex27 function together during peroxisome fission
Summary: Yeast peroxisome fission is mediated by the dynamin-related proteins Vps1 and Dnm1. Pex27 accumulates at peroxisomal membrane constrictions and is specifically required for Vps1 accumulation and activity on peroxisomes.
The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system
Summary: TRPML1 defects accompany abnormalities in the endolysosomal system in Alzheimer's disease and APOE-ε4 iPSC-derived neurons, which are recapitulated by neuronal PIKfyve inhibition and remediated by the TRPML1 agonist ML-SA1.
Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis
Summary: ZDHHC5 targets protocadherin 7 to the cell surface and cleavage furrow by a palmitoylation-dependent mechanism to promote successful cytokinesis in adherent mammalian cells.
The force-dependent filamin A–G3BP1 interaction regulates phase-separated stress granule formation
Summary: Interaction of G3BP1 with the mechanosensing domain repeat 21 of filamin A regulates phase-separated stress granule formation.
Aberrantly activated TAK1 links neuroinflammation and neuronal loss in Alzheimer's disease mouse models
Summary: TAK1, a protein kinase highly activated in the hippocampus of Alzheimer's disease model mice, kills neurons through necroptosis, leading to neuronal loss.
The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast
Summary: The genes of the fission yeast ecl family respond to various forms of starvation. Phosphate starvation induces ecl3+, which contributes to starvation-induced G1 arrest and sexual differentiation.
Rac1 and Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons
Summary: Rac1 and Rac3 severely affect cortical interneuron migration by affecting centrosome, Golgi and lysosome positioning. TPC2 likely contributes to the phenotype by decreasing axonogenesis and somatic migration.
Vps9d1 regulates tubular endosome formation through specific activation of Rab22A
Summary: We identify Vps9d1, a previously uncharacterized VPS9 protein, as a novel Rab22A-GEF that regulates both tubular endosome formation and cargo recycling in clathrin-independent endocytosis.
An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum
Summary: An N-acetyltransferase ortholog resides in the Plasmodium falciparum endoplasmic reticulum and is essential for parasite survival in red blood cells, but uninvolved in protein export.
Swip-1 promotes exocytosis of glue granules in the exocrine Drosophila salivary gland
Summary: Swip-1 regulates actomyosin activity upstream of Rho-GTPase signaling to drive proper vesicle membrane crumpling and expulsion of cargo.
LRRK1 functions as a scaffold for PTP1B-mediated EGFR sorting into ILVs at the ER–endosome contact site
Summary: LRRK1 facilitates dephosphorylation of EGFR by PTP1B, an ER-localized protein tyrosine phosphatase, at the ER–endosome contact site. This is required for EGFR sorting into ILVs and downregulation of EGFR signaling.
TOOLS AND RESOURCES
Shotgun knockdown of RNA by CRISPR-Cas13d in fission yeast
Summary: A new CRISPR-Cas13d-based toolkit was developed for efficient knockdown of RNA, and an effective guide RNA was designed to allow targeting of gfp and knockdown of transcripts carrying gfp.
A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
Summary: CRISPR-mediated gene disruption via indel mutations does not always ensure a complete gene knockout. Here, we describe an efficient and practical approach for producing complete gene knockout cell lines in human diploid cells.
FIRST PERSON
Interviews with Biologists @ 100 conference speakers

Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and Sadaf Farooqi, and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
The spatial choreography of mRNA biosynthesis

In their Review, André Ventura-Gomes and Maria Carmo-Fonseca detail the latest research progress and technological advancements that are helping to unlock how nuclear organisation underpins control of gene transcription and pre-mRNA splicing.
JCS-FocalPlane Training Grants

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction

In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.