Issues

Summary: A summary of how tethers play a key role in the recognition of specific cargo containers and in membrane fusion.

Summary: Recent discoveries have shown that the WH2-domain-based actin nucleator SPIRE has a role in actomyosin-driven transport processes involved in mammalian reproduction, skin pigmentation and wound healing.

Summary: Disturbed flow is a key factor in promoting endothelial dysfunction. Frizzled-4 mediates the pro-inflammatory and barrier-disrupting effects of disturbed flow in endothelial cells via a WNT5A- and β-catenin-dependent mechanism.

Summary: SARS-CoV2 infection neutralizes the pH of the ERGIC due to the proton channel activity of the viral envelope protein, which is prevented by mutations at Val25.

Summary: Yeast peroxisome fission is mediated by the dynamin-related proteins Vps1 and Dnm1. Pex27 accumulates at peroxisomal membrane constrictions and is specifically required for Vps1 accumulation and activity on peroxisomes.

Summary: TRPML1 defects accompany abnormalities in the endolysosomal system in Alzheimer's disease and APOE-ε4 iPSC-derived neurons, which are recapitulated by neuronal PIKfyve inhibition and remediated by the TRPML1 agonist ML-SA1.

Summary: ZDHHC5 targets protocadherin 7 to the cell surface and cleavage furrow by a palmitoylation-dependent mechanism to promote successful cytokinesis in adherent mammalian cells.

Summary: Interaction of G3BP1 with the mechanosensing domain repeat 21 of filamin A regulates phase-separated stress granule formation.

Summary: TAK1, a protein kinase highly activated in the hippocampus of Alzheimer's disease model mice, kills neurons through necroptosis, leading to neuronal loss.

Summary: The genes of the fission yeast ecl family respond to various forms of starvation. Phosphate starvation induces ecl3⁺, which contributes to starvation-induced G1 arrest and sexual differentiation.

Summary: Rac1 and Rac3 severely affect cortical interneuron migration by affecting centrosome, Golgi and lysosome positioning. TPC2 likely contributes to the phenotype by decreasing axonogenesis and somatic migration.

Summary: We identify Vps9d1, a previously uncharacterized VPS9 protein, as a novel Rab22A-GEF that regulates both tubular endosome formation and cargo recycling in clathrin-independent endocytosis.

Summary: An N-acetyltransferase ortholog resides in the Plasmodium falciparum endoplasmic reticulum and is essential for parasite survival in red blood cells, but uninvolved in protein export.

Summary: Swip-1 regulates actomyosin activity upstream of Rho-GTPase signaling to drive proper vesicle membrane crumpling and expulsion of cargo.

Summary: LRRK1 facilitates dephosphorylation of EGFR by PTP1B, an ER-localized protein tyrosine phosphatase, at the ER–endosome contact site. This is required for EGFR sorting into ILVs and downregulation of EGFR signaling.

Summary: A new CRISPR-Cas13d-based toolkit was developed for efficient knockdown of RNA, and an effective guide RNA was designed to allow targeting of gfp and knockdown of transcripts carrying gfp.

Summary: CRISPR-mediated gene disruption via indel mutations does not always ensure a complete gene knockout. Here, we describe an efficient and practical approach for producing complete gene knockout cell lines in human diploid cells.