Issues
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Cover image
Cover Image
Cover: Confocal microscopy image of wild-type Drosophila secretory salivary gland stained for F-actin (green), Swip-1 (magenta) and DAPI (blue). See article by F. Lehne and S. Bogdan (jcs260366).
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RESEARCH HIGHLIGHTS
EDITORIAL
CELL SCIENCE AT A GLANCE
Membrane tethers at a glance
Summary: A summary of how tethers play a key role in the recognition of specific cargo containers and in membrane fusion.
REVIEW
The role of SPIRE actin nucleators in cellular transport processes
Summary: Recent discoveries have shown that the WH2-domain-based actin nucleator SPIRE has a role in actomyosin-driven transport processes involved in mammalian reproduction, skin pigmentation and wound healing.
RESEARCH ARTICLES
Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway
Summary: Disturbed flow is a key factor in promoting endothelial dysfunction. Frizzled-4 mediates the pro-inflammatory and barrier-disrupting effects of disturbed flow in endothelial cells via a WNT5A- and β-catenin-dependent mechanism.
SARS-CoV-2 infection alkalinizes the ERGIC and lysosomes through the viroporin activity of the viral envelope protein
Summary: SARS-CoV2 infection neutralizes the pH of the ERGIC due to the proton channel activity of the viral envelope protein, which is prevented by mutations at Val25.
The dynamin-related protein Vps1 and the peroxisomal membrane protein Pex27 function together during peroxisome fission
Summary: Yeast peroxisome fission is mediated by the dynamin-related proteins Vps1 and Dnm1. Pex27 accumulates at peroxisomal membrane constrictions and is specifically required for Vps1 accumulation and activity on peroxisomes.
The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system
Summary: TRPML1 defects accompany abnormalities in the endolysosomal system in Alzheimer's disease and APOE-ε4 iPSC-derived neurons, which are recapitulated by neuronal PIKfyve inhibition and remediated by the TRPML1 agonist ML-SA1.
Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis
Summary: ZDHHC5 targets protocadherin 7 to the cell surface and cleavage furrow by a palmitoylation-dependent mechanism to promote successful cytokinesis in adherent mammalian cells.
The force-dependent filamin A–G3BP1 interaction regulates phase-separated stress granule formation
Summary: Interaction of G3BP1 with the mechanosensing domain repeat 21 of filamin A regulates phase-separated stress granule formation.
Aberrantly activated TAK1 links neuroinflammation and neuronal loss in Alzheimer's disease mouse models
Summary: TAK1, a protein kinase highly activated in the hippocampus of Alzheimer's disease model mice, kills neurons through necroptosis, leading to neuronal loss.
The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast
Summary: The genes of the fission yeast ecl family respond to various forms of starvation. Phosphate starvation induces ecl3+, which contributes to starvation-induced G1 arrest and sexual differentiation.
Rac1 and Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons
Summary: Rac1 and Rac3 severely affect cortical interneuron migration by affecting centrosome, Golgi and lysosome positioning. TPC2 likely contributes to the phenotype by decreasing axonogenesis and somatic migration.
Vps9d1 regulates tubular endosome formation through specific activation of Rab22A
Summary: We identify Vps9d1, a previously uncharacterized VPS9 protein, as a novel Rab22A-GEF that regulates both tubular endosome formation and cargo recycling in clathrin-independent endocytosis.
An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum
Summary: An N-acetyltransferase ortholog resides in the Plasmodium falciparum endoplasmic reticulum and is essential for parasite survival in red blood cells, but uninvolved in protein export.
Swip-1 promotes exocytosis of glue granules in the exocrine Drosophila salivary gland
Summary: Swip-1 regulates actomyosin activity upstream of Rho-GTPase signaling to drive proper vesicle membrane crumpling and expulsion of cargo.
LRRK1 functions as a scaffold for PTP1B-mediated EGFR sorting into ILVs at the ER–endosome contact site
Summary: LRRK1 facilitates dephosphorylation of EGFR by PTP1B, an ER-localized protein tyrosine phosphatase, at the ER–endosome contact site. This is required for EGFR sorting into ILVs and downregulation of EGFR signaling.
TOOLS AND RESOURCES
Shotgun knockdown of RNA by CRISPR-Cas13d in fission yeast
Summary: A new CRISPR-Cas13d-based toolkit was developed for efficient knockdown of RNA, and an effective guide RNA was designed to allow targeting of gfp and knockdown of transcripts carrying gfp.
A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
Summary: CRISPR-mediated gene disruption via indel mutations does not always ensure a complete gene knockout. Here, we describe an efficient and practical approach for producing complete gene knockout cell lines in human diploid cells.
FIRST PERSON
Call for papers: Cell and Tissue Polarity
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We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists

We are excited to announce the launch of The Forest of Biologists, a new biodiversity initiative created with support from the Woodland Trust, aiming to counteract nature loss and safeguard some of the most critically endangered ecosystems for future generations. For every Research Article and Review/Commentary article that is published in Journal of Cell Science, a native tree is planted in a forest in the UK. View our virtual forest here.
Propose a new Workshop for 2025

Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
Editorial: Publishing where it matters
Editor-in-Chief Michael Way outlines Journal of Cell Science’s plans for the upcoming year and introduces Seema Grewal as our new Executive Editor.
Cell Scientists to Watch

As a community-focused journal, Journal of Cell Science is keen to support the next generation of cell biologists. Check out Cell Scientists to Watch, our interview series featuring talented researchers who have recently set up their own labs.