Issues

Summary: In this Review we briefly summarize the basis for the development of nanobodies and provide an overview of the multiple applications of these versatile binders in biological research.

Summary: SETD2 catalyses trimethylation of lysine 36 on histone H3. Here, we review the roles of SETD2 in mammals, with a focus on the central nervous system, and summarise the consequences of pathogenic SETD2 variants in humans.

Summary: Fission yeast Pik1 localizes exclusively to the trans-Golgi independently of Ncs1, where it contributes to PI4P but not PI(4,5)P₂ synthesis. Pik1 does not affect cytokinesis.

Highlighted Article: In C. elegans, nuclear closure around spindle microtubules requires BAF-1 binding to LEM-domain proteins and reveals redundant and unique functions for EMR-1, LEM-2 and CHMP-7 in nuclear maintenance.

Highlighted Article: Saturated fats elicit muscle cell-autonomous dysregulation of the basal-state machinery required for the GLUT4 translocation that ‘primes’ skeletal muscle cells for insulin resistance.

Summary: Defects in centrosome organization (centriole number and length) are imparted by MCPH-associated mutations in the CPAP G-box domain, which impacts spindle organization and cell survival.

Summary: Centrosome amplification is sufficient, without additional pro-oncogenic alterations, to drive early tumorigenic change in a normal breast epithelial background.

Summary: Identification of a SUN-like protein in the apicomplexan parasite Toxoplasma gondii that is essential for replication.