Issues
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Cover image
Cover Image
Cover: Minimal tags for fluorescent labeling of transmembrane axon initial segment components in living neuroblastoma cell lines and primary neurons. Genetically encoded unnatural amino acids and click chemistry were used to label recombinant NF186 and NaV1.6 with small fluorescent dyes. The cover image shows click-labeled NF186 in neuroblastoma cells. Cells expressing three different NF186 constructs, each labeled at one specific position, are color-coded in different colors and overlaid in one image. See article by N. Stajković et al. (jcs260600).
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RESEARCH HIGHLIGHTS
CELL SCIENCE AT A GLANCE
Viral evasion of the interferon response at a glance
Summary: We highlight the major tactics employed by viruses to evade or antagonize cellular machineries and pathways that sense viral nucleic acid and elicit antiviral interferon responses.
REVIEW
Emerging roles of p300/CBP in autophagy and autophagy-related human disorders
Summary: We review the function of p300/CBP in autophagy, its regulation by cellular pathways and small molecules, and the role of p300/CBP-regulated autophagy in human disorders.
RESEARCH ARTICLES
The cytoplasmic tail of the mechanosensitive channel Pkd2 regulates its internalization and clustering in eisosomes
Summary: The fission yeast polycystin Pkd2 promotes cell proliferation. We discovered that the cytoplasmic tail of this essential ion channel promotes its intracellular trafficking and prevents its clustering in the plasma membrane.
Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP
Summary: The HAUS complex is a direct target of the Ran-GTP pathway, suggesting that branching microtubule nucleation is directly regulated by the Ran-GTP gradient around chromosomes in dividing cells.
SCG10 is required for peripheral axon maintenance and regeneration in mice
Highlighted Article: Knockout mouse studies of SCG10, a regulator of microtubule dynamics in neurons, highlight the importance of SCG10-mediated microtubule dynamics for peripheral axon maintenance and regeneration.
Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and the vacuole
Highlighted Article: Ivy1 interacts with Rab7-like protein Ypt7 and PI(3)P, and is a novel substrate of the vacuolar casein kinase Yck3. Ivy1 phosphorylation determines its localization and function at endosomes and vacuoles.
Transport of synaptic vesicles is modulated by vesicular reversals and stationary cargo clusters
Summary: Simulations of axonal transport in C. elegans neurons suggest that long-lived stationary vesicle clusters modulate net cargo flow, and that vesicle reversals regulate the proportion of stationary clusters and moving vesicles.
Divergent functions of histone acetyltransferases KAT2A and KAT2B in keratinocyte self-renewal and differentiation
Summary: A distinct gene regulatory mechanism exists in which histone acetyltransferases KAT2A and KAT2B drive divergent functions of self-renewal and differentiation in human keratinocytes, with implications for cutaneous wound healing.
Optogenetically engineered Ca2+ oscillation-mediated DRP1 activation promotes mitochondrial fission and cell death
Summary: Optogenetically driven Ca2+ oscillation promotes mitochondrial fission by increasing phosphorylation at Ser616 of DRP1 via the activation of CaMKII, ERK and CDK1.
EPLIN-β is a novel substrate of ornithine decarboxylase antizyme 1 and mediates cellular migration
Summary: EPLIN-β is degraded by ornithine decarboxylase antizyme 1 (Az1) in a proteasome-mediated, ubiquitination-independent manner. Az1 absence leads to elevated EPLIN-β levels, causing enhanced cellular migration.
Vinculin Y822 is an important determinant of ligand binding
Summary: Vinculin Y822 modulates ligand binding to control focal adhesion morphology and tumorigenicity in cancer cells.
TOOLS AND RESOURCES
Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry
Summary: By using genetically encoded unnatural amino acids and click chemistry, we developed minimally invasive tags for live-cell labeling of large neuronal transmembrane proteins and ion channels.
Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families
Highlighted Article: Endocytic recycling pathways control cellular processes including cell migration. Here, we identify protein cargoes and trafficking machinery associated with the major recycling regulators Rab4a, Rab11a and Rab25.
Development of a fluorescence reporter system to quantify transcriptional activity of endogenous p53 in living cells
Summary: Tumor suppressor protein p53 is one of the hub factors of the cellular stress responses. Our novel reporter system allows easy and precise visualization of the transcriptional activity of endogenous p53.
FIRST PERSON
JCS fast-track option
Have a paper that has been reviewed elsewhere? JCS is pleased to consider such manuscripts for fast-tracked decision making. Send us your manuscript together with the full set of reviews and decision letters, and we will make an initial decision within one week.
Special Issue – Cell Biology of Mitochondria
Our special issue on ‘Cell Biology of Mitochondria’ is now complete. Explore this issue and read the Editorial from our Guest Editors Ana J. García-Sáez and Heidi McBride.
Save the date – Imaging Cell Dynamics
We are delighted to announce that we will be hosting a 2026 Imaging Cell Dynamics meeting. This meeting will provide a unique opportunity to bring together experts working at the interface between cell biology and imaging. Save the date for 11-14 May 2026 and register for more information.
Origin and evolution of mitochondrial inner membrane composition
In this Review, Kailash Venkatraman and colleagues provide an examination of the morphological similarities between prokaryotic intracytoplasmic membranes and mitochondrial inner membranes, and whether cristae evolution has driven specialisation of the mitochondrial lipidome.
Resolution in super-resolution microscopy
Super-resolution microscopy (SRM) has emerged as a powerful tool for biological discovery. In this Perspective, Kirti Prakash and colleagues compile expert opinions on crucial, yet often overlooked, aspects of SRM that are essential for maximising its benefits and advancing the field.
