Issues

Summary: Exploration of the various factors within a biospecimen that can derail the accuracy of quantitative fluorescence microscopy, as well as approaches to identify and mitigate these problems.

Summary: We discuss the roles of the retrograde motor proteins dynein and myosin VI in the regulation of endocytic pathways, focusing on structural and mechanistic details of these motors and their adaptors.

Summary: A summary of the history and progress in our understanding of Ena/VASP family proteins, which constitute crucial members of actin filament polymerases operating in various cell types, tissues and processes.

Summary: The photopigment melanopsin (OPN4), which provides inner retinal photoreception in mammals, shows light-dependent activation of G_s G protein that is more pronounced for mouse than human photopigment.

Summary: Membrane-associated SARS-CoV-2 ORF6 does not require localization to the nuclear envelope to block nuclear trafficking and antagonize interferon, raising the possibility it has additional functions within membrane networks.

Summary: Osp94, a member of the Hsp110/Sse1 family of heat-shock proteins, acts as a holdase and that the C-terminal region plays a PA28α-like role in the refolding of unfolded proteins.

Summary: The Legionella effector Lpg1137 targets membrane compartments such as ER, mitochondria-associated membrane and mitochondria through binding to phosphatidic acid.

Highlighted Article: We provide insights into nanoscale mechanotransduction processes at the nuclear envelope and the role of emerin oligomerization at the LINC complex for maintaining nuclear integrity during mechanical challenges to cells.

Highlighted Article: Dynamic lateral protrusions from neuroepithelial apical endfeet form a lattice that connects non-neighbouring cells, is regulated during neurogenesis and may provide a basis for signalling across epithelia.

Summary: ER-PM tethering at close distance limits Ca²⁺ entry by confining STIM-ORAI complexes to the periphery of contact sites.

Summary: SPAG1 scaffolds R2TP-like complexes to assist formation of dynein subunit complexes in dynein assembly; however, a 60-kDa isoform can assemble outer dynein arms in the absence of full-length SPAG1.

Highlighted Article: We demonstrate the existence of endogenous nuclear actin filaments in healthy mammalian oocytes, and that these F-actin structures deteriorate with advancing reproductive age.

Summary: The endolysosomal ion-channel TRPML1 is a regulator of mitochondrial function; an isoform-selective activator modulates TRPML1 channel activity and potentially could treat liver cancer.

Summary: In mouse oocytes, a CDC25B requirement for meiotic maturation is associated not only with the initial CDK1 activation and resumption of meiosis but also with the meiosis I-meiosis II transition.

Summary: A novel genetic and biochemical strategy reveals that heparan sulfate modification is necessary and sufficient for the key modulatory function of the GPC3 glypican in the Hedgehog signaling pathway.

Summary: We developed luminescent tools to evaluate inhibitors of breast cancer progression. Castanospermine inhibits breast cancer cell invasion and slightly suppresses cancer cell migration.

Summary: Loss of ezrin expression leads to suppression of procaterol-stimulated ciliary beating without changes to ciliary morphology and basal body orientation in mouse airway epithelium.

Highlighted Article: Ribosomal sequences demonstrate a unique spatial organization in early mouse embryos. Inhibition of their transcription disturbs this organization but also affects the transcription of major satellite repeats.

Summary: A description of RNA-assisted sequestration of TIA1 and other RNA-binding proteins by TDP-35 and live-cell imaging for dynamic sequestration of TDP-43.

Summary: Our study identified HuR as a regulator involved in the apoptosis induced by dsRNA or virus, and illustrated it acts through binding to BCL2 mRNA and suppressing its translation.

Summary: A series of in vitro experiments with an Lmbr1l knockout mouse model reveal that LMBR1L acts as a negative regulator of Norrin/β-catenin signaling in mammalian retinal vascular development.

Summary: H₂O₂ and Shh signaling engage in a regulatory crosstalk during fin regeneration: H₂O₂ controls Shh expression and Shh regulates H₂O₂ levels, via different mechanisms in successive stages.

Summary: High-content biosensor imaging with kinase translocation reporters reveals substantial cell-to-cell heterogeneity in kinase activity driven by G-protein-coupled receptors.