CELL SCIENTISTS TO WATCH
Summary: An overview of centrosome 3D organization, describing molecular asymmetries between the mother (mature) and the daughter (immature) centrioles, and how these are acquired in synchrony with the cell cycle.
Summary: Integrin α6β4 is recruited with vimentin to perinuclear focal and fibrillar adhesions indicating how integrin α6β4 cooperates with other integrins to drive migration and invasive growth.
Summary: Endothelial cells heterogeneity is key to complex collective functions and cell behaviour. We developed a novel image-based endothelial cell profiling tool and quantified heterogeneity in NOTCH signalling in monolayers.
Highlighted Article: The histone demethylase DPY-21 has catalytic and non-catalytic roles in condensin DC-mediated X chromosome repression. The non-catalytic activity regulates dynamics of condensin DC binding to X chromosomes.
Summary: The oncoprotein PRL regulates epithelial cell density by driving apoptosis at confluence, which is important for convergent extension during early development in vertebrate embryos.
Multiple regions within junctin drive its interaction with calsequestrin-1 and its localization to triads in skeletal muscle
Summary: We describe the molecular interactions of the junctin luminal tail with the macromolecular complex that regulates Ca2+ release from the sarcoplasmic reticulum in skeletal muscle.
Contribution of protein–protein interactions to the endothelial-barrier-stabilizing function of KRIT1
Highlighted Article: Contrary to current models, KRIT1 does not require binding to ICAP1α and Rap1, or junctional localization, to stabilize the endothelial barrier. Instead, self-interaction and/or β1 integrin activation are critical elements of KRIT1 signaling.
Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins
Summary: Alzheimer's disease-associated isoform APOE4 accumulates in lysosomes and dysregulates autophagic flux. APOE can enter the lysosome through autophagy requiring LAMP2A, a receptor for chaperone-mediated autophagy.
Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos
Summary: Chiffon within the Gcn5-containing CHAT complex plays key roles in embryonic gene expression and histone H3 acetylation in Drosophila.
Highlighted Article: SAF-A contributes to the formation of open chromatin structure. We have discovered that SAF-A supports DNA replication at multiple steps.
Highlighted Article: The ubiquitin ligase APC/C is required for anaphase execution and localizes to the centrosome during mitosis. Its function is to remove Cep152, an inhibitor of microtubule nucleation, from the centrosome.
CPVT-associated calmodulin variants N53I and A102V dysregulate Ca2+ signalling via different mechanisms
Summary: CaM mutations associated with human cardiac arrhythmia syndromes result in CaM dysfunction, which appears to be a key driver of the disease, with different mutants acting via different mechanisms.
Summary: Expression, mutagenic and cytological analyses identify cyclophilin A as an important regulator of Twist1 phosphorylation and stability that acts as an inhibitor of A549 cell apoptosis.
MITOL-mediated DRP1 ubiquitylation and degradation promotes mitochondrial hyperfusion in a CMT2A-linked MFN2 mutant
Summary: Mitochondrial hyperfusion caused by the CMT2A-linked R364W mutation of MFN2 is the result of altered interactions between R364W-MFN2 and MITOL, which lead to enhanced proteasomal degradation of DRP1.