Issues

Summary: The features of chromosome folding and organization are important for proper nuclear function. This Cell Science at a Glance article reviews chromosome and genome organization dynamics through the cell cycle.

Summary: Overview of the recent studies defining the role of actin cytoskeleton during cilia formation in polarized epithelial cells.

Highlighted Article: A protein kinase in the cytoplasm is phosphorylated within 1 min after engagement of ciliary receptors during Chlamydomonas fertilization and is required to trigger the fusion-essential increase in cytoplasmic cAMP.

Summary: Using a buckling model, we extend the current description of local podosome protrusion and include a mechanical explanation for protrusion on the cell scale.

Summary: This study reports that suspended tumor cells respond to fluid shear stress through histone acetylation-mediated nuclear expansion, which protects CTCs from shear-induced apoptosis.

Summary: A chemical probe-based activity assay was used to show that inhibition of tripeptidyl peptidase II (TPPII) by serpinB2 regulates proteostasis in senescent cells.

Summary: Interaction of cytosolic terminal regions in the Jen1 carboxylate transporter, belonging to the major facilitator superfamily, are critical for trafficking and transport activity.

Summary: We show that yeast ESCRT-III interacts with Vps68, an endosomal membrane protein. We propose that Vps68 and ESCRT-III cooperate in the abscission of intraluminal vesicles by weakening the lipid bilayer.

Highlighted Article: Characterization of new roles for retromer in C. elegans development and in the regulation of ciliogenesis in mammalian cells, suggesting a novel role for retromer in CP110 removal from the mother centriole.

Summary: Characterization at a single-cell level of the unique heterogeneity of the mammary epithelial Comma-1D cell line, which illustrates its use as an experimental model to study both differentiation and transformation.

Summary: General Image Analysis of Nuclei-based Images (GIANI) is a new plugin for the popular FIJI platform, designed for the automated analysis of 3D microscopy images of a wide range of sample types.