Cover: An image of HeLa cell plated on the substrate coated by an animal lectin, galectin-8. F-actin is labelled in green and myosin-IIA in magenta. Galectin-8 promotes the formation of numerous filopodia and lamellipodia in adherent cells, but not the actomyosin bundles usually found in cells spreading on substrates coated by fibronectin. See article by W. Li et al. (jcs252221).
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CELL SCIENTISTS TO WATCH
CRISPR-Cas tools to study gene function in cytokinesis
Summary: This Review highlights recent advances and questions remaining in the field of cytokinesis, and how gene editing tools will facilitate significant advances in the field.
The role of nuclear Ca2+ in maintaining neuronal homeostasis and brain health
Summary: This Review discusses the ‘nuclear calcium hypothesis’ in the context of human brain function, the role of nuclear Ca2+ in controlling diverse forms of neuroadaptation and neuroprotection, and its dysregulation in disease.
Emerging evidence for kindlin oligomerization and its role in regulating kindlin function
Summary: In this Review, we present an update of recent progress in obtaining kindlin structures, and the implication for integrin activation based on formation of kindlin oligomerization as well as the regulation of this process.
Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals
Summary:Drosophila Dock likely functions downstream of WASp and the Arp2/3 complex to regulate the size and stability of the germline ring canals in the developing egg chamber.
De novo formation of early endosomes during Rab5-to-Rab7a transition
Highlighted Article: A crucial step in endosomal maturation is the exchange of Rab5 with Rab7a, and we show that this two-phase exchange is finalized by the formation of Rab5-positive early endosomes.
Differential cellular responses to adhesive interactions with galectin-8- and fibronectin-coated substrates
Summary: Fibronectin and galectin-8 have dramatically different effects on cell spreading and focal adhesion formation; however, when mixed, they synergize in stimulating filopodia formation.
Ribosome quality control activity potentiates vaccinia virus protein synthesis during infection
Summary: The ribosome-associated quality control factor ZNF598, which senses ribosome collisions, is a host factor necessary for vaccinia viral protein synthesis.
Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1
Summary: The autophagy proteins TMEM41B and VMP1 belong to the DedA superfamily, whose members are predicted to have ion-coupled transporter-like structures containing two facing reentrant loops.
Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood–brain barrier
Highlighted Article: Ultrastructural analysis of T cell migration across the blood–brain barrier (BBB) under physiological flow identifies BBB tricellular junctions as sites of T cell diapedesis.
The formin inhibitor SMIFH2 inhibits members of the myosin superfamily
Highlighted Article: The inhibitor SMIFH2 inhibits a variety of myosins almost as efficiently as formins, its designated targets.
AKR1B10 negatively regulates autophagy through reducing GAPDH upon glucose starvation in colon cancer
Summary: NRP1 can regulate AKR1B10 expression, and AKR1B10 interacts with and inhibits the nuclear importation of GAPDH to repress autophagy upon glucose starvation.
α-Arrestin ARRDC3 tumor suppressor function is linked to GPCR-induced TAZ activation and breast cancer metastasis
Summary: The α-arrestin ARRDC3 is a multi-functional tumor suppressor that distinctly regulates GPCR trafficking and TAZ-dependent Hippo signaling in metastatic triple negative breast cancer.
Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L
Summary: Tyrosine sulfation and O-glycosylation in the N-terminus of the chemoattractant receptor GPR15 oppositely regulate the binding of its ligand GPR15L and receptor signaling.
Identification of a novel Bax–Cdk1 signalling complex that links activation of the mitotic checkpoint to apoptosis
Highlighted Article: Bax-mediated translocation of Cdk1–cyclin B to the mitochondrion signals cell death and is the cytoplasmic signal that connects mitotic cell cycle arrest to apoptosis.
TOOLS AND RESOURCES
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish
Summary: A robust and flexible approach to understand tissue-specific gene functions in vivo is established that allows efficient gene disruption, gene-specific rescue and biosensor imaging in the knockout background.
JCS Journal Meeting 2023: Imaging Cell Dynamics
Our 2023 Journal Meeting on ‘Imaging Cell Dynamics’ will be held from 14-17 May 2023 in Lisbon, Portugal. Due to popular demand, we can currently only accept applications for online attendance. Apply now to attend this meeting virtually. Registration deadline: 31 March.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
Editorial: Publishing where it matters
Editor-in-Chief Michael Way outlines Journal of Cell Science’s plans for the upcoming year and introduces Seema Grewal as our new Executive Editor.
preLights 5th Birthday webinar
preLights, our preprint highlighting service, is celebrating its 5th birthday this year. To mark the occasion, join us online on 14 March 2023 at 16:00 GMT for a discussion, led by four preLights alumni, on how to identify and navigate the challenges and opportunities while shaping your career as an early-career researcher.
Cell Scientists to Watch
As a community-focused journal, Journal of Cell Science is keen to support the next generation of cell biologists. Check out Cell Scientists to Watch, our interview series featuring talented researchers who have recently set up their own labs.