CELL SCIENTISTS TO WATCH
Summary: We question how soluble factors are asymmetrically partitioned across the nuclear pore complex to establish the directional basis of nucleocytoplasmic transport.
Summary: This Review highlights the diverse functions of myosin, kinesin and dynein motor proteins in the transport and positioning, fission and fusion, and turnover of mitochondria.
Summary: Leukocytes seem to prefer specific sites for crossing the endothelium. These are referred to as transmigration hotspots. This Review highlights the potential mechanisms and tissue specificity of these transmigration hotspots.
Highlighted Article: The p97 AAA-ATPase and adaptor UBXN1 recognize ubiquitylated, misfolded proteins and deliver them to the aggresome for degradation. Loss of p97-UBXN1 inhibits aggresome formation and disposal of Huntingtin inclusion bodies.
Natural killer cell immune synapse formation and cytotoxicity are controlled by tension of the target interface
Highlighted Article: Analyses of the impact of altered target rigidity reveal that soft targets expressing activating ligands do not induce polarisation of natural killer cellular machinery required for cytolysis.
DNAJB chaperones suppress destabilised protein aggregation via a region distinct from that used to inhibit amyloidogenesis
Summary: DNAJB chaperones potently suppress destabilised luciferase aggregation in cells. For DNAJB6 and DNAJB8 this requires a region in the C-terminal domain different from that used to inhibit amyloidogenesis.
Summary: Loss of function of transcription co-factor LBH caused progressive hair cell loss in adult mice, likely mediated by dysregulation of genes promoting hair cell survival and DNA repair.
Summary: The metabolic sensor protein kinase AMPK is a key regulator of acute retinal vascular permeability by activating endothelial nitric oxide synthase and p38 MAPK.
Summary: Tiam1 maintains centriole number and ensures correct chromosome segregation via interaction with βTRCP, part of the E3 ligase complex, which degrades PLK4, the master regulator of centriole duplication.
Summary:Drosophila Importin-9 loss disrupts female and male meiosis. Importin-9 also functions during the histone to protamine transition and in nuclear transport of proteasome proteins during spermiogenesis.
Tumor microenvironment acidity modulates ROR1 to promote epithelial–mesenchymal transition and hepatocarcinoma metastasis
Highlighted Article: An acidic microenvironment plays a key role in hepatocarcinoma metastasis by increasing ROR1 expression, providing a promising target for tumor management.
Rab5 regulates macropinocytosis by recruiting the inositol 5-phosphatases OCRL and Inpp5b that hydrolyse PtdIns(4,5)P2
Summary: A series of mutagenesis experiments reveals that Rab5 supports macropinosome scission by promoting PtdIns(4,5)P2 hydrolysis, through the recruitment of 5-inositol phosphatases to circular ruffles.
PAPOLA contributes to cyclin D1 mRNA alternative polyadenylation and promotes breast cancer cell proliferation
Summary: Poly(A)polymerase α upregulates the expression of cyclin D1 and enhances proliferation of breast cancer cells. It could be a therapeutic target in triple-negative breast cancer.
Summary: Axon regeneration drives selective localization of alternatively spliced Cdc42 mRNA isoform to PNS axons.
Summary: The use of simulations and experiments to illustrate the advantage imparted by combined heterogeneity in cell size and deformability in enhancing cancer invasiveness.
TOOLS AND RESOURCES
Summary: We developed an artificial Rab11-specific binding domain, named RBD11, that can be used for visualizing and temporally analyzing endogenous, active Rab11 in mammalian cultured cells.
Development of conformational BRET biosensors that monitor ezrin, radixin and moesin activation in real time
Summary: Characterization of BRET-based conformational biosensors that monitor individual ezrin, radixin or moesin activation at the plasma membrane in living cells.