Cover: Winning image, ‘Trippy Tension Turtle Tailing Treats’, from the one-year anniversary FocalPlane image competition held in July 2021 taken by Tejeshwar Rao, postdoctoral fellow at Mattheyses Research Group, UAB School of Medicine, Birmingham, Alabama, USA. The image is an artistically rendered montage of HUVECs plated on a multiplex Tension Gauge Tether (TGT) surface (12+56 pN). The glow edges depict the tension generated following probe opening with growth factor stimulation, captured using total internal reflection fluorescence microscopy.
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CELL SCIENTISTS TO WATCH
The Autophagy, Inflammation and Metabolism Center international eSymposium – an early-career investigators’ seminar series during the COVID-19 pandemic
Summary: We report on the Autophagy, Inflammation and Metabolism Center eSymposium 2020 for early career investigators (ECIs), describe its impact on ECI careers and provide a post-pandemic world meetings formula.
Managing risky assets – mitophagy in vivo
Summary: This Review explores the in vivo roles of mitophagy in mammals, focusing on the best-studied examples – mitophagy in neurodegeneration, cardiomyopathy, metabolism and red blood cell development – to draw out common themes.
Downregulation of the Tem1 GTPase by Amn1 after cytokinesis involves both nuclear import and SCF-mediated degradation
Summary: The Tem1 GTPase, which is critical for mitotic exit and cytokinesis, is doubly inactivated at the end of mitosis by the Amn1 protein.
Tyrosine kinase-independent actions of DDR2 in tumor cells and cancer-associated fibroblasts influence tumor invasion, migration and metastasis
Summary: The collagen receptor DDR2, a receptor tyrosine kinase, exhibits tyrosine kinase-independent functions that influence paracrine regulation of breast tumor cell invasion and breast cancer metastasis.
A cell-based drug discovery assay identifies inhibition of cell stress responses as a new approach to treatment of epidermolysis bullosa simplex
Summary: High-content screening of an epidermolysis bullosa simplex cell model identifies inhibition of EGFR signalling as a mechanism that can reduce mutant keratin aggregate formation and improve intercellular cohesion.
The TPR- and J-domain-containing proteins DJC31 and DJC62 are involved in abiotic stress responses in Arabidopsis thaliana
Highlighted Article: DJC31 and DJC62 localize to the ER where they interact with cytosolic HSP70 and HSP90. Their loss in Arabidopsis results in sensitivity to osmotic stress and abscisic acid.
Fission yeast TOR complex 1 phosphorylates Psk1 through an evolutionarily conserved interaction mediated by the TOS motif
Summary: By analyzing S6 kinase in fission yeast, we have demonstrated that the TOR signaling (TOS) motif-mediated substrate recognition by TOR complex 1 is conserved from yeast to humans.
Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay
Summary: We describe an imaging-based assay to detect protein–protein interactions in fission yeast that can be used for cytosolic proteins, nuclear proteins and components of multiprotein complexes.
Local DNA synthesis is critical for DNA repair during oocyte maturation
Summary: The DNA synthesis machinery is essential to repair the low levels of DNA damage induced by ROS in oocyte reserve.
Cdc42 reactivation at growth sites is regulated by local cell-cycle-dependent loss of its GTPase-activating protein Rga4 in fission yeast
Highlighted Article: Resumption of polarized Cdc42 activation at growth sites depends on a cell-cycle cue. This is facilitated by the loss of a Cdc42 GAP, Rga4, from growth sites at the end of cell division.
AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors
Summary: The assembly of the AP1 adaptor complex is a highly orchestrated process controlled by AAGAB.
TOOLS AND RESOURCES
Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography
Highlighted Article: We demonstrate that ptychographic hard X-ray tomography is a powerful scanning tool that can image retina tissue at sub-200 nm resolution for analysis of neural degeneration processes.
Direct observation of aggregate-triggered selective autophagy in human cells
Highlighted Article: We describe an assay that enables live-cell imaging of the autophagic turnover and degradation of inducible protein aggregates in conjugation with key players in the autophagy pathway.
Python-Microscope – a new open-source Python library for the control of microscopes
Summary: An open-source Python library for accessing a range of hardware devices used in microscopy.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing in Journal of Cell Science - read more about why you should choose JCS or visit our submission page now.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists
The Forest of Biologists is a biodiversity initiative created by The Company of Biologists, with support from the Woodland Trust. For every Research and Review article published in Journal of Cell Science a native tree is planted in a UK forest. In addition to this we are protecting and restoring ancient woodland and are dedicating these trees to our peer reviewers. Visit our virtual forest to learn more.
Propose a new Workshop for 2025
Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
FocalPlane Image Competition 2023
FocalPlane’s 2023 image competition is underway, in celebration of the community site’s third birthday. Click here for submission details and information on how to showcase your favourite image.