CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Investigating the morphological dynamics of the plasma membrane by high-speed atomic force microscopy
Summary: A summary of recent advancements in live-cell imaging using high-speed atomic force microscopy, with a special emphasis on its application to morphological dynamics of the plasma membrane during endocytosis.
Highlighted Article: Transient SUMOylation of RepoMan controls the recruitment of lamin A to telophase chromosomes, lamin A dephosphorylation and nuclear lamina formation.
Summary: TANGO1 is required to maintain the fundamental organization of the early secretory pathway in mammalian cells to support secretion of a diverse range of newly synthesized cargo proteins.
Phosphorylation of the AMPA receptor subunit GluA1 regulates clathrin-mediated receptor internalization
Summary: During synaptic plasticity to enhance synaptic strength, phosphorylation of AMPA receptor subunit GluA1 at serine 845 reduces receptor internalization by inhibiting interactions with endocytic proteins.
ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms
Summary: We report a novel, non-canonical role for ADAR1 as a negative regulator of stress granules that has implications for the role of ADAR1 in the antiviral response.
Activation of IRE1, PERK and salt-inducible kinases leads to Sec body formation in Drosophila S2 cells
Summary: In S2 cells, the phase-separated Sec bodies form upon the combined activation of salt-inducible kinases, IRE1 and PERK.
Highlighted Article: Downregulation of the single C. elegans lamin gene increases animal size and fat content specifically in dietary restricted animals. The lamin protein acts in the mTOR pathway to regulate these phenotypes.
Highlighted Article: Liquid–liquid phase separation of oncogenic fusion proteins could be a potential mechanism for aberrant transcriptional activation.
Nuclear translocation of the tagged endogenous MAPK MPK-1 denotes a subset of activation events in C. elegans development
Highlighted Article: Tagged endogenous C. elegans MPK-1 shows activation-dependent cytosol-to-nuclear translocation. This tool provides novel insights into MPK-1 localization compared with other markers of in vivo ERK activation.
Summary: Contractile signaling promotes stabilization of E-cadherin to enforce orderly epithelial migration.
Phosphorylated cingulin localises GEF-H1 at tight junctions to protect vascular barriers in blood endothelial cells
Summary: Vascular leak in response to histamine, thrombin and VEGF can be counteracted by AMPK phosphorylating cingulin at its head domain. Consequential binding of GEF-H1 to tight junctions protects vascular barrier function.
TOOLS AND RESOURCES
Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor
Summary: The dT-2xrGBD location-based Rho biosensor relocalizes more efficiently than other sensors of this type, and this sensor enables the observation of endogenous Rho activity in cultured cells.