CELL SCIENTISTS TO WATCH
Electrically tunable lenses – eliminating mechanical axial movements during high-speed 3D live imaging
Summary: Electrically tunable liquid lenses possess shape-changing principles. We discuss their recent applications, limitations and emerging solutions in enabling high-speed cost-effective 3D live microscopy.
Phosphorylation in the intrinsically disordered region of F-BAR protein Imp2 regulates its contractile ring recruitment
Summary: Phosphorylation of the F-BAR protein Imp2 within its intrinsically disordered region modulates its localization and function at the contractile ring.
LFA-1 and kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors
Summary: LFA-1 and kindlin-3 play unconventional roles in the dynein-dependent and talin-1-independent centripetal transport of actomyosin-associated SLP-76 microclusters and in TCR-proximal signaling pathways.
Distinct temporal expression of the GW182 paralog TNRC6A in neurons regulates dendritic arborization
Summary: Developmentally regulated expression of the miRISC protein GW182 plays an important role in determining dendritic arborization by regulating somatodendritic translation and actin cytoskeletal dynamics.
Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe
Summary: We report a comparative analysis of self-assembled spindles and canonical centrosomal spindles in fission yeast, which could clarify the mechanisms underlying acentrosomal meiosis.
A three-dimensional spheroid-specific role for Wnt–β-catenin and Eph–ephrin signaling in nasopharyngeal carcinoma cells
Summary: We report a new 3D spheroid model of Epstein–Barr virus-positive and -negative nasopharyngeal carcinoma that resembles advanced and metastatic tumors and has potential use in drug target identification.
The Bardet–Biedl syndrome complex component BBS1 controls T cell polarity during immune synapse assembly
Highlighted Article: The Bardet–Biedl syndrome complex component BBS1 regulates proteasome-dependent F-actin clearance from the centrosome to enable its translocation to the T cell immune synapse.
A polycystin-2 protein with modified channel properties leads to an increased diameter of renal tubules and to renal cysts
Summary: Replacement of the pore region of polycystin-2 with that of polycystin-2L1 results in wider renal tubules and polycystic kidney disease, thus demonstrating the essential function of its ion channel properties.
A developmental stage- and Kidins220-dependent switch in astrocyte responsiveness to brain-derived neurotrophic factor
Summary: Here, we describe how astrocytes respond differently to stimuli of growth factor during development and the postnatal stage, and the role of the Kidins220 protein in this process.
Summary: Eukaryotic cells need large protein complexes to produce microtubules. We studied the regulation of nuclear and septum-associated microtubule-organizing complexes, and their coordination in one cell.
EVI/WLS function is regulated by ubiquitylation and is linked to ER-associated degradation by ERLIN2
Highlighted Article: The WNT secretory factor EVI/WLS is ubiquitylated and linked to ER-associated degradation by multiple proteins, providing insight into the link between WNT signalling and the ubiquitin–proteasome system.
Highlighted Article: We report a genetically encoded fluorescent reporter sensitive to all known activators of GPR68, a G-protein-coupled receptor involved in many important physiological functions.
Summary: There is an unexpected role for the alternative splicing factor PfSR1 in activating the DNA damage response in the human malaria parasite Plasmodium falciparum.