CELL SCIENCE AT A GLANCE
Summary: A discussion of the roles of the spindle midzone, a microtubule-containing structure that connects the two half-spindles during mitosis, contributes to chromosome segregation and specifies the location of cytokinetic furrowing.
Summary: Spatiotemporally regulated posttranslational modifications of light intermediate chain subunits could modulate the diverse functional spectrum of cytoplasmic dynein through selective adaptor engagement.
Summary: We survey mechanisms for regulating crossover interference during meiosis, and review how studies of phase separation, DNA repair and pan-nuclear signaling may resolve longstanding mysteries in the field.
Summary: The nuclear scaffold protein lamin A/C modulates nuclear stiffness, and heterogeneous lamin A/C density leads to diverse nuclear strain under stress.
Highlighted Article: Endocytic delivery to lysosomes by kiss and run/membrane fusion entails pore formation commencing at the edge of tether arrays, and demonstrates SNARE redundancy and compensation.
Highlighted Article: Chemically induced MT asters interacting with kinesin-5 motors spontaneously form optimal packing patterns in vivo. A computational model reproduces the experimental patterns and predicts their scaling.
Integrity of the short arm of the nuclear pore Y-complex is required for mouse embryonic stem cell growth and differentiation
Highlighted Article: Seh1 and Nup43, although dispensable in pluripotent mouse ESCs, are required for normal cell growth, viability upon differentiation and maintenance of proper nuclear pore complex density.
Enrichment of Zα domains at cytoplasmic stress granules is due to their innate ability to bind to nucleic acids
Summary: We identify the interacting partners of the ZαβDAI–RNA complex in vivo and show that their translocation to stress granules is mainly mediated by the nucleic acid-binding ability of the Zα domains.
Highlighted Article: The heat shock induces the translocation of long noncoding RNA MALAT1 from nuclear speckles to a heat-inducible noncoding RNA-containing nuclear body (HiNoCo body).
TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose
Summary: Nitrogen-dependent localization of S. pombe Ght5 on the cell surface is maintained by TORC2, which inhibits α-arrestin Aly3-dependent ubiquitylation and subsequent vacuolar translocation of Ght5.
Dissecting the PRSS37 interactome and potential mechanisms leading to ADAM3 loss in PRSS37-null sperm
Summary: PRSS37 interactome analysis showed that PRSS37 specifically modulates PDILT and ADAM3 interaction, which is a prerequisite for ADAM3 transport from the endoplasmic reticulum to the germ cell surface.
Hherisomes, Hedgehog specialized recycling endosomes, are required for high level Hedgehog signaling and tissue growth
Summary: Ultrastructural identification of specialized recycling endosomes that contain Hedgehog and sustain physiological Hedgehog activity for patterning and tissue growth in the wing disc.
Summary: Recruitment of the host cell Golgi by the parasitophorous vacuolar membrane of Plasmodium berghei is crucial for optimal pre-erythrocytic development. The interaction is accompanied by parasite-induced formation of miniaturized host cell Golgi.
Summary: An in-depth cellular and molecular characterization of myosin XIX (Myo19)-deficient cells reveals that different cellular and molecular processes are linked to mitochondrial dynamics regulated by Myo19.