Issues

Summary: A review of studies on target of rapamycin complex 2 in budding yeast suggesting that the primordial function of this kinase is to maintain the biophysical properties of the plasma membrane in a narrow, optimal window.

Summary: Peroxisome biogenesis and disease: VDAC2-BAK mediating Catalase export, a new mutation of Pex26 in PBD, a GTP provider DNNAMO1, and impaired BDNF-TrkB signaling in PBDs.

Summary: In this Review, we revisit the non-canonical roles of caveolins in a variety of cellular contexts including liver, brain, lymphocytes, cilia and cancer cells, as well as consider insights from invertebrate systems.

Summary: CRISPR-Mb3Cas12a can target a broader range of sequences in murine zygotes compared to AsCas12a and LbCas12a, and has a lower frequency of large on-target insertions or deletions than seen with Cas9 and high overall knock-in efficiency.

Summary: The small G-protein Ran is an important non-centrosomal microtubule regulator in neurons, and the active GTP-bound Ran is transported by actin waves to its target region.

Summary: Phosphorylation of the linker domain causes EB1 to adopt a more curved structural conformation and disrupts the high-affinity binding of EB1 to microtubule growing ends.

Summary: The kinesin-1 motor protein plays a key role in controlling the membrane exchanges necessary for the intracellular survival of Salmonella. The complexity of kinesin-1 regulation by Salmonella effector proteins is highlighted.

Summary: Human pulmonary artery endothelial cells from male and female donors differ in their proliferative potential, a finding that could explain the higher incidence of pulmonary arterial hypertension in women.

Summary: Analysis of MT1-MMP phosphorylation and cell surface expression reveals a role for β1 integrin signaling during invadopodia formation and cell invasion.

Summary: Combined inhibition of Arg and MAP4K4 by bosutinib reduces turnover of integrin-based focal adhesions. This provides robust protection against inflammation-induced vascular leakage.

Highlighted Article: CLIC4 regulates remodeling of the sub-plasma-membrane actomyosin network within the cytokinetic furrow by recruiting MST4 kinase and regulating ezrin phosphorylation, thus regulating cortex stiffness and blebbing.

Summary: EML4–ALK fusions are major drivers of lung cancer. Particular fusion variants act through a novel actionable pathway involving NEK7 and NEK9, promoting cell migration and potentially influencing metastatic progression.

Highlighted Article: SET removes histone H1 from chromatin. When SET is depleted, the resulting reduction in histone H1 removal leads to enhanced resistance to various types of DNA lesions.

Summary: Nutrition starvation stress induces a spatiotemporal interaction of SNAP29 with the cytokeratin network, which mediates the assembly of CTP synthase filaments along the cytokeratin network to modulate enzymatic activity.

Summary: Apoptosis was induced by osmotic shrinkage while keeping monovalent ions at fixed concentrations. It was found that shrinkage itself, rather than potassium loss or cytoskeleton-mediated effects, is responsible for apoptosis.

Highlighted Article: Most integrins can bind several ligands. We show how one integrin, αVβ3, selects between two different ligands, fibronectin and vitronectin, based on force-regulated conformational changes in αVβ3 integrin.

Summary: Hypomethylation by 5-Aza-CdR upregulates the SIPA1 expression and promotes epithelial–mesenchymal transition in breast cancer cells, possibly increasing the risk of cancer cell metastasis.

Highlighted Article: A dual functioning DNA polymerase in African trypanosomes supports DNA replication and distribution of mitochondrial DNA progeny.