Issues

Summary: The activity of the critical oncoprotein RAS can be negatively regulated by two groups of proteins, GAPs and RASSF proteins, as reviewed here.

Summary: Different cryo-EM approaches, ranging from analysis of isolated molecules to in situ imaging, have yielded many new insights into the main gate of the secretory pathway.

Highlighted Article: Karyopherins confer onto nuclear pore complexes a retention mechanism that maintains the steep GTPase Ran gradient across the nuclear envelope.

Summary: The tubulin modification polyglutamylation regulates the overall motility of different types of transport cargoes in axons of hippocampal neurons, and is thus likely to act as a general transport control mechanism in neurons.

Highlighted Article: The vesicle-tethering and fusion factors Boi1 and Boi2 are shown to initiate the formation of actin filaments by recruiting the Bni1–Bud6 actin nucleation complex to sites of exocytosis.

Highlighted Article: The focal adhesion protein DLC1 is a direct transcriptional target of YAP/TAZ and TEAD, and is crucial for YAP-driven collective migration and sprouting angiogenesis in endothelial cells.

Summary: Analysis in Tetrahymena thermophila reveals remarkable expansion of oligomeric protein complexes that organize the interactions between endosomes, lysosomes and related compartments in ciliates.

Highlighted Article: Live-cell imaging of endogenously tagged formins coupled with examination of formin-null lines reveals that phylogenetically distinct formins differentially contribute to actin formation in plant cells.

Summary: The deubiquitylase USP9X promotes efficient homologous recombination DNA repair through BRCA1 and RAD51 regulation independently of its catalytic activity.

Highlighted Article: CENP-T plays a critical role in mitosis. Here, we show that CENP-T regulates both meiotic resumption and anaphase entry in meiotic oocytes, which is substantially different from the function of CENP-T in mitosis.

Summary: GDE2, a six-transmembrane GPI-specific phospholipase, undergoes constitutive endosomal recycling via newly identified sequences and a unique leucine residue in its C-terminal tail as a way to regulate neuronal differentiation.

Summary: Schizosaccharomyces pombe DNA translocases Rrp1 and Rrp2 modulate nucleosome dynamics to maintain centromere and telomere function, and dysregulation of their activity leads to genome instability.

Summary: Although Rho GTPases are well-characterized activators of serine/threonine protein kinase Pak1, cooperative recruitment of Arl4A and Pak1 to the plasma membrane promotes Pak1 activation independently of Rac1-Pak1 signaling.