Issues
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Cover image
Cover Image
Cover: Growth-arrested mammalian cells display reduced mitochondrial dynamics and a concomitant decrease in mitochondrial association with the endoplasmic reticulum. This de-tethering of these organelles ultimately causes reduced compartment-specific activity of mTORC1 complex, leading to polysomal retention and reduced export of miRNAs. The 3D rendering of mitochondrial structures from a proliferating HeLa cell (yet to reach growth arrest) shown here depicts the highly branched mitochondrial network in a gradient of dark pink to off-white according to individual strand lengths. The blue to green colour marks the degree of association of the mitochondria and endoplasmic reticulum at specific points. See article by S. Chatterjee et al. (jcs250241).
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RESEARCH HIGHLIGHTS
STICKY WICKETS
OBITUARY
FIRST PERSON
CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Tumour-directed microenvironment remodelling at a glance
Summary: An outline of the processes by which epithelial tumours regulate their tumour microenvironment, and how new insights into tumour–microenvironment interactions have led to ideas on targeting it as treatments.
REVIEWS
The journey of Ca2+ through the cell – pulsing through the network of ER membrane contact sites
Summary: Traveling through the ER, Ca2+ is transported towards membrane contact sites with mitochondria or endolysosomes, where it plays key roles in the regulation of essential organellar biology, which are reviewed here.
Notch in mechanotransduction – from molecular mechanosensitivity to tissue mechanostasis
Summary: This Review presents evidence that Notch signaling is directly and indirectly mechanosensitive, and discusses engineered and computational approaches to help unravel its complex dynamics.
RESEARCH ARTICLES
Sec71 separates Golgi stacks in Drosophila S2 cells
Summary: Golgi stacks are scattered in the cytoplasm of Drosophila cells. Sec71 is required for separating Golgi stacks, as its impairment, for example, through brefeldin A (BFA) treatment, produces Golgi aggregations termed BFA bodies.
Mitochondria control mTORC1 activity-linked compartmentalization of eIF4E to regulate extracellular export of microRNAs
Summary:Mitochondrial activity and tethering with the ER are linked to compartmentalization of the translation initiation factor eIF4E to the polysomes, which controls miRNA-mediated repression and miRNA export.
A novel mitosis-specific Cep215 domain interacts with Cep192 and phosphorylated Aurora A for organization of spindle poles
Summary: A mitosis-specific centrosome-targeting domain of Cep215 binds Cep192 and phosphorylated Aurora A to play a role in maintaining the structural integrity of spindle poles during mitosis.
Retromer retrieves the Wilson disease protein ATP7B from endolysosomes in a copper-dependent manner
Summary: The Wilson disease protein ATP7B utilizes lysosomal exocytosis to export excess copper in hepatocytes. The retromer complex retrieves ATP7B from endolysosomes and recycles it back to the trans-Golgi network.
Disrupting polycystin-2 EF hand Ca2+ affinity does not alter channel function or contribute to polycystic kidney disease
Summary: A refutation of a common hypothesis regarding Ca2+ control mechanisms of polycystin-2 – a primary cilia ion channel, the dysregulation of which causes autosomal dominant polycystic kidney disease.
EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration
Summary: Constitutively active and truncated EGFR transcriptionally represses integrins via a SHC–MEK signaling axis, and communicates with neighboring cells through TNFα to promote cooperative invasion.
TrkB deubiquitylation by USP8 regulates receptor levels and BDNF-dependent neuronal differentiation
Summary: USP8 interacts with and deubiquitylates TrkB neurotrophin receptors, regulating levels of both total and activated receptors and BDNF-dependent differentiation of hippocampal neurons.
CD301 mediates fusion in IL-4-driven multinucleated giant cell formation
Highlighted Article: CD301, a C-type lectin domain family 10 member, is driven by IL-4 and mediates macrophage fusion for the formation of multinucleated giant cells.
ESCRT recruitment by the S. cerevisiae inner nuclear membrane protein Heh1 is regulated by Hub1-mediated alternative splicing
Highlighted Article: Heh1-S, the inner nuclear membrane protein resulting from Hub1-mediated splicing of HEH1 pre-mRNA, contributes to nuclear envelope maintenance by preventing excessive recruitment of the ESCRT adaptor Chm7.
The budding yeast Start repressor Whi7 differs in regulation from Whi5, emerging as a major cell cycle brake in response to stress
Highlighted Article: Cells can use the interplay between functionally redundant but differentially regulated cell-cycle repressors in order to confer new repression capabilities and to respond to specific cellular conditions.
A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast
Highlighted Article: Yeast Mdh2 is dually localized to the cytosol and peroxisomes, and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism.
Sequential peripheral enrichment of H2A.Zac and H3K9me2 during trophoblast differentiation in human embryonic stem cells
Highlighted Article: As early as 12 hours after embryonic stem cells begin differentiating to trophoblasts, acetylated H2A.Z becomes enriched at the nuclear periphery; we find that loss of H2A.Z acetylation is followed sequentially by enrichment of dimethylated H3K9.
TOOLS AND RESOURCES
Trackosome: a computational toolbox to study the spatiotemporal dynamics of centrosomes, nuclear envelope and cellular membrane
Summary: Development of a new image analysis toolbox called Trackosome and its use to study nuclear envelope oscillations during early mitosis and correlations between the trajectories of the centrosomes.
CORRECTION
Interviews with Biologists @ 100 conference speakers

Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and Sadaf Farooqi, and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
The spatial choreography of mRNA biosynthesis

In their Review, André Ventura-Gomes and Maria Carmo-Fonseca detail the latest research progress and technological advancements that are helping to unlock how nuclear organisation underpins control of gene transcription and pre-mRNA splicing.
JCS-FocalPlane Training Grants

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction

In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.