Issues

Summary: A guide on how to appropriately design hypothesis-driven, quantitative fluorescence microscopy experiments using a reverse logic.

Summary: A close look at recent studies on FGF2 and IL-1β that produced breakthrough discoveries revealing the molecular mechanisms of unconventional protein secretion in mammalian cells.

Summary: A review of how ERp44 is key in sculpting the composition of the secretome and of the organelles of the early secretory pathway.

Summary: Mitochondrial survivin, which is found exclusively in cancer cells, prevents removal of defective mitochondria, impairing respiration; cancer cells respond by switching to glycolysis to survive.

Summary: During cytokinesis, non-muscle Myosin II controls centralspindlin maintenance at the cleavage furrow and actomyosin ring constriction by recruiting the PI(4)P-binding protein GOLPH3.

Summary: CKAP4 plays a role in maintaining mitochondrial functions through the binding to VDAC2 at ER–mitochondria contact sites in a palmitoylation-dependent manner.

Summary: RNF144a-mediated VRK3 degradation under oxidative stress leads to persistent ERK activation and promotes apoptosis.

Summary: Autophagy-dependent formation of an extensive Syntaxin17-marked tubular network synchronizes degradative activity across a broad region of remodeling muscle during Drosophila development.

Highlighted Article: S-acylation of the tumour suppressor protein sprouty-2 requires specific cysteine and charged residues in the cysteine-rich domain, and regulates plasma membrane targeting.

Highlighted Article: In yeast, FIT2 homologs play a dual role in maintaining cellular proteostasis and membrane lipid homeostasis. The unfolded protein response is necessary to replace their function if they are ablated.

Highlighted Article: PBAC5 is an evolutionarily distinct chaperone that forms a tripartite complex with PBAC1 and PBAC2 to promote assembly of the 20S proteasome α-ring in Arabidopsis.

Highlighted Article: The mitotic checkpoint protein Mad1 is required for Nup153 recruitment to anaphase chromatin and, in turn, for post-mitotic but not interphase nuclear pore complex assembly.

Highlighted Article: By using C. elegans epidermis as a model, we find that periodically organized subcellular structures perform synchronized duplication to increase their numbers and accommodate epidermal cell growth.

Summary: Using LUHMES, a ciliated human neuronal cell model, the underlying ‘neurobiology’ of cilia and ciliopathies can be investigated along a complete time line of neuron differentiation.