CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Summary: A hitchhiker's guide to the rapidly expanding YAP/TAZ regulatory network, centered on the Hippo pathway and on mechanosensing of the physical properties of the cell microenvironment.
Summary: Microtubules are important regarding various steps of protein transport. Regulation of their functions is achieved by protein interactions but also by post-translational modifications of tubulin.
Summary: A Review of the diverse roles of chloride ions in physiology, the current repertoire of fluorescent chloride reporters, and a discussion with biologists about the nuances of using them.
Summary: Morgana, a Drosophila homologue of an oncogene, physically interacts with Hsp90 complexes and has an independent function in regulating microtubule polymerisation, both in vitro and in vivo.
Summary: The deubiquitylase Usp14 is required for maintenance of the equilibrium between free and covalently linked ubiquitin moieties during later stages of Drosophila spermatogenesis.
Capacitation-associated alkalization in human sperm is differentially controlled at the subcellular level
Summary: Human sperm display differential pHi regulation at the subcellular level upon capacitation, in a process involving PKA signaling and several membrane transport proteins and culminating in hyperactivation.
Summary: A range of biochemical and biophysical methods, including FLIM/FRET, reveal the mechanisms of SWAP-70 dimerization and F-actin binding both in vitro and in living cells.
Senescent mesenchymal stem cells remodel extracellular matrix driving breast cancer cells to a more-invasive phenotype
Highlighted Article: Senescence-induced biophysical changes in mesenchymal stem cells enabled them to develop a matrix-remodeling phenotype, which promoted increased cancer cell proliferation and invasion.
A GTPase-induced switch in phospholipid affinity of collybistin contributes to synaptic gephyrin clustering
Summary: This study shows that the small GTPase TC10 modulates the phosphoinositide-binding-specificity of collybistin, which regulates collybistin trafficking to inhibitory postsynapses and thus affects the assembly of the synaptic gephyrin scaffold.
Plasma membrane processes are differentially regulated by type I phosphatidylinositol phosphate 5-kinases and RASSF4
Summary: PIP5KIβ and PIP5KIγ synthesize PtdInsP2; RASSF4 stimulates PIP5KIγ. These three proteins have different effects on a panel of functional assays, implying multiple pools of lipid.
LAX28 is required for the stable assembly of the inner dynein arm f complex, and the tether and tether head complex in Leishmania flagella
Summary: The inner dynein arm f (or l1) complex is required for coordinating flagellar motility. We show that LAX28 is needed for its function and localization in the flagellum of Leishmania mexicana.
AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells
Highlighted Article: The neuronal endocytic adaptor protein AP180 has a dual role in the clearance of release sites and in membrane retrieval in inner hair cells of the murine cochlea.
TOOLS AND RESOURCES
Highlighted Article: A new method to produce eukaryotic actin isoforms with distinct post-translational modifications, which will greatly facilitate biochemical, biophysical and structural studies.
Summary: Introduction to an easy to implement microscopy-based platform enabling accurate quantification of fast endocytic recycling, biosynthetic delivery and protein turnover in real time.