Issues

Summary: A hitchhiker's guide to the rapidly expanding YAP/TAZ regulatory network, centered on the Hippo pathway and on mechanosensing of the physical properties of the cell microenvironment.

Summary: Microtubules are important regarding various steps of protein transport. Regulation of their functions is achieved by protein interactions but also by post-translational modifications of tubulin.

Summary: A Review of the diverse roles of chloride ions in physiology, the current repertoire of fluorescent chloride reporters, and a discussion with biologists about the nuances of using them.

Summary: Morgana, a Drosophila homologue of an oncogene, physically interacts with Hsp90 complexes and has an independent function in regulating microtubule polymerisation, both in vitro and in vivo.

Summary: The deubiquitylase Usp14 is required for maintenance of the equilibrium between free and covalently linked ubiquitin moieties during later stages of Drosophila spermatogenesis.

Summary: Human sperm display differential pH_i regulation at the subcellular level upon capacitation, in a process involving PKA signaling and several membrane transport proteins and culminating in hyperactivation.

Summary: A range of biochemical and biophysical methods, including FLIM/FRET, reveal the mechanisms of SWAP-70 dimerization and F-actin binding both in vitro and in living cells.

Highlighted Article: Senescence-induced biophysical changes in mesenchymal stem cells enabled them to develop a matrix-remodeling phenotype, which promoted increased cancer cell proliferation and invasion.

Summary: This study shows that the small GTPase TC10 modulates the phosphoinositide-binding-specificity of collybistin, which regulates collybistin trafficking to inhibitory postsynapses and thus affects the assembly of the synaptic gephyrin scaffold.

Summary: PIP5KIβ and PIP5KIγ synthesize PtdInsP₂; RASSF4 stimulates PIP5KIγ. These three proteins have different effects on a panel of functional assays, implying multiple pools of lipid.

Summary: The inner dynein arm f (or l1) complex is required for coordinating flagellar motility. We show that LAX28 is needed for its function and localization in the flagellum of Leishmania mexicana.

Highlighted Article: The neuronal endocytic adaptor protein AP180 has a dual role in the clearance of release sites and in membrane retrieval in inner hair cells of the murine cochlea.

Highlighted Article: A new method to produce eukaryotic actin isoforms with distinct post-translational modifications, which will greatly facilitate biochemical, biophysical and structural studies.

Summary: Introduction to an easy to implement microscopy-based platform enabling accurate quantification of fast endocytic recycling, biosynthetic delivery and protein turnover in real time.