Summary: We discuss perspectives on the mechanisms of formation of Wnt- and Hedgehog-containing extracellular vesicles, and their functions in development and various human pathologies.
Summary: This Review explores the emerging role of the biophysical properties of the basement membrane in regulating critical aspects of vascular health and disease.
Summary: In this Review, we highlight recent work identifying non-transcriptional mechanisms crucial to regulating the core mammalian clock proteins and their role in circadian rhythmicity.
Summary: In vitro F-actin gliding assays and TIRF microscopy reveal that myosin flattens the Arp2/3-dependent actin branches up to the point of them breaking, and reduces the probability that new branches are formed.
Phosphoregulation of the cytokinetic protein Fic1 contributes to fission yeast growth polarity establishment
Summary: Complex phosphoregulation of the S. pombe contractile ring protein Fic1 influences polarity establishment and the transition from a unicellular state into a state with invasive pseudohyphal growth.
Increased supraorganization of respiratory complexes is a dynamic multistep remodelling in response to proteostasis stress
Summary: Cells remodel respiratory complex organization depending on metabolic status. Here, using a two-dimensional complexome profiling strategy, we propose a biphasic mechanism for this remodelling during proteostasis stress.
Intraflagellar transport during assembly of flagella of different length in Trypanosoma brucei isolated from tsetse flies
Summary: Investigation of intraflagellar transport in several trypanosome developmental stages extracted from tsetse flies provides new insights into mechanisms controlling flagellum length.
Highlighted Article: The β1 integrin interactor Rabgap1 promotes recycling of active β1 integrins from endosomes to enhance cell migration on 2D surfaces as well as cell invasion into a 3D matrix.
Role of a versatile peptide motif controlling Hox nuclear export and autophagy in the Drosophila fat body
Summary: A generic Hox peptide works as an unconventional nuclear export signal (NES) to control Hox nuclear export and developmental autophagy in the Drosophila larval fat body.
A discrete interface in matrix stiffness creates an oscillatory pattern of endothelial monolayer disruption
Highlighted Article: Transitions in matrix stiffness create oscillatory patterns of endothelial monolayer barrier disruption and cell contractility.
Mitochondrial import, health and mtDNA copy number variability seen when using type II and type V CRISPR effectors
Summary: Testing the targeting and tolerance of CRISPR effectors and gRNAs in mammalian cell lines towards the design of functional MitoCRISPR tools.
Highlighted Article: Using endogenous tagging and interaction proteomics, we identified the ER-associated lipid droplet biogenesis factor ACSL3 as a GABARAPL2-binding partner that serves as novel regulator of the enigmatic UFM1 conjugation pathway.
APC/CCdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit
Summary: APC/CCdh1 ubiquitylates both borealin, via a non-canonical degron motif, and Aurora B to terminate activity of the CPC in G1 phase. Disruption of this ubiquitylation reduces the efficiency of DNA replication.
Summary: The non-fusogenic Sec22b–Stx1 SNARE complex contributes to plasma membrane expansion by interacting with the E-Syt lipid transfer proteins at ER–PM contact sites.
Microtubule polyglutamylation is important for regulating cytoskeletal architecture and motility in Trypanosoma brucei
Summary: An imbalance of microtubule polyglutamylation leads to the disintegration of the posterior cytoskeleton and alterations of the swimming behaviour from a directional into a non-directional, diffusive mode in Trypanosoma brucei.
Summary: The mitochondrial fission protein dynamin-related protein 1 (DRP1) plays an essential role in early transcriptional reprogramming during beige and brown adipocyte differentiation.
TOOLS AND RESOURCES
Summary: A new methodology to synchronize RPE-1 cells in mitosis for large-scale biochemical and proteomics studies.