Issues
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Cover image
Cover Image
Cover: Epifluorescence image showing the Giardia lamblia microtubule cytoskeleton in a CRISPRi knockdown of the disc-associated protein DAP5188. The microtubule cytoskeleton, including the ventral disc, eight flagella and median body, is visualised in the knockdown strain through expression of mNeonGreen-tagged β-tubulin (green). See article by C. Nosala et al. (jcs227355).
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RESEARCH HIGHLIGHTS
STICKY WICKET
FIRST PERSON
MEETING REPORT
CELL SCIENCE AT A GLANCE
Membrane trafficking in the retinal pigment epithelium at a glance
Summary: This Cell Science at a Glance article focuses on membrane trafficking events in retinal pigment epithelial cells, which are uniquely specialised to fulfil the multiple functions of this epithelium in supporting vision.
REVIEW
Diverse cellular functions of barrier-to-autointegration factor and its roles in disease
Summary: BAF (BANF1) orchestrates key cellular processes including cell division, repair of nuclear ruptures, and anti-viral intrinsic immunity.
RESEARCH ARTICLES
The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways
Summary: Transient interaction between RhoU and ITSN2 on a subset of vesicles regulates cargo recycling.
Disc-associated proteins mediate the unusual hyperstability of the ventral disc in Giardia lamblia
Highlighted Article: Identification of over 50 disc-associated proteins associated with the ventral disc in G. lamblia, two of which mediate microtubule hyperstability or limit microtubule dynamics.
Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1
Summary: CTR1 (SLC31A1) is the only known mammalian importer of copper. We show that CTR1 is a retromer complex cargo protein, and that retromer is required for cellular sensitivity to extracellular copper.
The lncRNA MEG3 mediates renal cell cancer progression by regulating ST3Gal1 transcription and EGFR sialylation
Summary: The lncRNA MEG3 mediates renal cell cancer progression. MEG3 regulates ST3Gal1 expression through interaction with the c-Jun transcription factor, with downstream effects on EGFR sialylation and PI3K–AKT signaling.
Lymphocytes perform reverse adhesive haptotaxis mediated by LFA-1 integrins
Summary: Like mesenchymal cells, lymphocytes perform adhesive haptotaxis, although adhesion is dispensable for amoeboid migration. Moreover, unlike mesenchymal cells, lymphocytes display a unique ability to follow decreasing gradients of adhesion.
The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment
Highlighted Article: An acidic patch in the kinetochore-targeting domain of CENP-E is required for association with the C terminus of BubR1 at kinetochores to facilitate chromosome alignment during mitosis.
BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation
Summary: GDF5 mutants were used to investigate the role of BMPR1A and BMPR1B in chondrogenesis and osteogenesis. We found that BMPR1A is necessary for chondrogenesis and osteogenesis, and BMPR1B prevents hypertrophy.
The Na+ pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization
Summary: Coincidence detection of phosphorylated and ubiquitylated cargo-specific regions is used for epsin target recognition among multiple ubiquitylated proteins in yeast.
TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis
Highlighted Article: ER transmembrane protein TMEM147 impacts on cellular cholesterol homeostasis via physical and functional interactions with LBR and DHCR, two key sterol reductases in cholesterol biosynthesis.
The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
Highlighted Article: A 3D structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions.
Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast
Highlighted Article: The absence of Pex24 or Pex32, two Hansenula polymorpha ER proteins, disturbs peroxisome–ER contact sites and leads to multiple peroxisomal defects, which can be restored using an artificial tether protein.
Call for papers - Cilia and Flagella: from Basic Biology to Disease
We are welcoming submissions for our upcoming special issue: Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Extended submission deadline: 31 March 2025.
History of our journals
As our publisher, The Company of Biologists, turns 100 years old, read about Journal of Cell Science’s journey and explore the history of each of our sister journals: Development, Journal of Experimental Biology, Disease Models & Mechanisms and Biology Open.
Introducing our new Associate Editors
In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
Diversity of microtubule arrays in animal cells at a glance
In this Cell Science at a Glance article, Emma van Grinsven and Anna Akhmanova provide an overview of the diverse microtubule arrays present in differentiated animal cells and discuss how these arrays form and function.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 7 March 2025 (decision by week commencing 21 April 2025) and 6 June 2025 (decision by week commencing 28 July 2025).
