Issues

Summary: A practical guide to organising sustainable and inclusive virtual seminar series.

Summary: We review plant meiosis, including chromosome tethering at the nuclear periphery that, we propose, defines chromosome dynamics-facilitating DNA sequence-based pairing of homologues

Summary: A Review of the multitude of extracellular proteoglycans, glycan-binding lectins and secreted enzymes regulating trans-synaptic signaling at the Drosophila neuromuscular junction.

Summary: A review of how IRE1α, the most conserved UPR sensor, controls cell fate and several cellular processes through the engagement of a signaling platform named the UPRosome.

Summary: Computational modeling shows that LRP6 endocytosis in vertebrates is clathrin-independent and not restricted to lipid rafts or microdomains and further suggests that signalosome formation within microdomains delays LRP6 internalization.

Highlighted Article: Crosstalk between cell adhesion dynamics and actomyosin regulates epithelial cell shape. The p120-catenin protein facilitates this crosstalk through modulation of Arf1 and RhoA signalling.

Summary: CRISPR/Cas9-mediated knockout reveals the molecular hierarchy of the PRDM14, CBFA2T2, CtBP1/2 and PRC2 complex, which are required for the transcriptional repression in embryonic stem cells.

Summary: The acidic domain of CENP-B facilitates assembly of various proteins at centromeric satellite DNA, including HP1 and ASH1L. CENP-B controls centromere epigenetic status.

Summary: miR-206 regulates the slow contractile properties of skeletal and cardiac muscle. miR-206 deletion shifts slow muscles to a faster profile and results in cardiac dysfunction specifically in male mice.

Summary: A poorly characterized phospholipid flippase in S. cerevisiae is involved in establishing phosphatidylserine asymmetry at the plasma membrane and has a role in the control of pseudohyphal growth.

Highlighted Article: Asymmetry and remodeling of the membrane skeleton might enable spatiotemporal modulation of membrane mechanics during distinct developmental events. C. elegans spectrin binds to ankyrin, and these proteins function to regulate neural development.

Summary: Aggregation-prone proteins associated with neurodegenerative diseases impair or evade the heat shock response in neuronal cells.

Summary: Non-muscle myosin II isoforms and myosin 18a have differing roles in coordinating cell-cortex tension release and myelin membrane expansion during oligodendrocyte development.

Summary: The motility and localization of melanosomes in the retinal pigment epithelium are dependent on cytoplasmic dynein and myosin-7a, thus supporting a capture mechanism that involves both microtubules and actin motors.

Summary: Two-dimensional affinity measurements of a T-cell receptor binding its agonistic peptide major histocompatibility complex molecule and the influence of cellular context.

Highlighted Article: Retromer, a master controller of endosomal cargo sorting, is deregulated in neurodegenerative disease. Here, we develop and apply a retromer knocksideways methodology to quantify endosomal cargo sorting upon acute perturbation.

Highlighted Article: At developing Xenopus neuromuscular junctions, the proteolytic activity of neuronal MT1-MMP digests the surrounding ECM proteins and mediates Lrp4 cleavage to regulate agrin deposition and signaling during presynaptic differentiation, respectively.

Summary: We present detailed novel quantitative imaging protocols, and review pharmacological and genetic manipulation for the investigation of clathrin-mediated endocytosis at multiple scales in Arabidopsis plants.