CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Summary: Overview of the selective autophagy of both pathogens and immunoregulators, highlighting a few examples that illustrate molecular strategies developed by pathogens to manipulate selective autophagy for their own benefit.
Summary: In this Review, we discuss how autophagy impacts on endocytosis, how endocytosis regulates autophagy in mammalian cells and how both pathways impact on quality control of the lysosome.
F-BAR domain protein Syndapin regulates actomyosin dynamics during apical cap remodeling in syncytial Drosophila embryos
Summary: Syndapin, an F-BAR domain protein, regulates the transition from Arp2/3-dependent protrusion remodeling and cap expansion to actomyosin-dependent cap buckling in the Drosophila blastoderm embryo.
Compartmentalization of adenosine metabolism in cancer cells and its modulation during acute hypoxia
Summary: The identification of redundant pathways that tightly control cellular ATP and adenosine levels provides novel insights into the purinergic mechanisms governing cancer cell metabolism and signaling.
Highlighted Article: The ubiquitin-conjugating enzyme Ubc13–Mms2 contributes to membrane protein sorting in the multivesicular body pathway, thus constituting an additional source of K63-polyubiquitylation beyond Rsp5/NEDD4 in the endocytic compartment.
The Lumiptosome, an engineered luminescent form of the apoptosome can report cell death by using the same Apaf-1 dependent pathway
Summary: We show for the first time that a modified version of Apaf-1 forms an apoptosome-like structure that triggers cell death by using a mechanism that is identical to that used by native Apaf-1
The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
Summary: We have identified an evolutionarily conserved nuclear lamina binding domain in the PRR14 nuclear lamina–heterochromatin tethering protein.
Summary: CSAG1, a novel regulator of mitosis that constrains the pericentriolar material to prevent spindle pole fragmentation, particularly, in cells that lack p53 function.
Sirtuin-1 regulates organismal growth by altering feeding behavior and intestinal morphology in planarians
Summary: Sirtuins regulate organismal growth in planarians by altering animal behavior and disrupting the differentiation of cellular progenitors.
Primary myeloid cell proteomics and transcriptomics: importance of β-tubulin isotypes for osteoclast function
Summary: Analysis of proteomic and transcriptomic data of primary myeloid cells reveals that the β-tubulin isotype Tubb6 participates in osteoclast podosome patterning, sealing zone structure and resorption activity.
En bloc TGN recruitment of Aspergillus TRAPPII reveals TRAPP maturation as unlikely to drive RAB1-to-RAB11 transition
Summary: Live microscopy of Aspergillus Golgi cisternae refutes the possibility that maturation of TRAPPI/TRAPPIII into TRAPPII mediates the transition between RAB1 and RAB11 that takes place across the Golgi.
Maturation and phenotype of pathophysiological neuronal excitability of human cells in tau-related dementia
Summary: Pathophysiological excitability was revealed for human cells in dementia. This provides a major advance in understanding of tau-induced brain neuropathology and sheds new light on human models of dementia.
Summary: Two cytoplasmic proteins are shown to be required for paraflagellar rod formation, which are extra-axonemal structures found in the flagella of trypanosomes.
A new brain mitochondrial sodium-sensitive potassium channel: effect of sodium ions on respiratory chain activity
Summary: A newly identified sodium-sensitive potassium channel in brain mitochondria might be involved in regulation of the respiratory chain.
LDL uptake-dependent phosphatidylethanolamine translocation to the cell surface promotes fusion of osteoclast-like cells
Summary: Uptake of low-density lipoprotein (LDL) through the LDL receptor is required for translocation of phosphatidylethanolamine to the cell surface via ATP binding cassette G1 transporter and the cell–cell fusion of osteoclast-like cells.
STRIPAK–PP2A regulates Hippo-Yorkie signaling to suppress retinal fate in the Drosophila eye disc peripodial epithelium
Summary: STRIPAK–PP2A promotes peripodial cell fate in the developing Drosophila eye epithelium by antagonizing the Hippo signaling axis, thereby promoting Yorkie activity and suppressing retinal cell fate.
A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion in S. cerevisiae
Highlighted Article: The characterization of auxiliary factors in sister chromatid cohesion as identified by genetic interaction mapping in yeast, reveals novel roles for the prefoldin complex and the microtubule-associated protein Irc15.
Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomyces pombe
Highlighted Article: Translesion synthesis polymerases are required for meiotic survival in fission yeast, and their loss disrupts meiotic chromosome segregation and Rec8 dynamics. Replication and DSB repair are unaffected.
DE-cadherin and Myosin II balance regulates furrow length for onset of polygon shape in syncytial Drosophila embryos
Summary: DE-cadherin and Myosin II balance regulates an optimal lateral furrow length for transition of a circular to a polygonal plasma membrane organization in cortical syncytial division cycles in Drosophila embryogenesis.
Mitochondrial–nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites
Highlighted Article: A live-cell assay to monitor heme distribution kinetics identifies GTPases in control of mitochondrial–ER contact sites and dynamics as being critical modulators of heme trafficking.
Canonical nucleators are dispensable for stress granule assembly in Drosophila intestinal progenitors
Highlighted Article: Previously defined canonical stress granule factors are not required for SG assembly in adult Drosophila progenitor cells, suggesting that other cell populations employ a similar noncanonical assembly mechanism in vivo.
TOOLS AND RESOURCES
Micro-stepping extended focus reduces photobleaching and preserves structured illumination super-resolution features
Summary: We present an upgrade to spinning disk confocal microscopes that dramatically improves photon balance and imaging speed for various imaging applications in projections, while simultaneously preserving super-resolution features.