Cover: Engineered PEG-4MAL hydrogel promotes formation of organized epithelial tubules. Fluorescence microscopy images of inner medullary collecting duct (IMCD) tubules within engineered hydrogel stained for actin (phalloidin, red) and nuclei (DAPI, blue) at day 21 after 3D encapsulation. This fully synthetic matrix addresses major limitations of biological materials, and its modular nature allows the characterization of the elementary matrix property contributions to renal organ development. See article by R. Cruz-Acunña et al. (jcs226639).
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CELL SCIENCE AT A GLANCE
Adaptor protein complexes and disease at a glance
Summary: Adaptor protein complexes select cargo for transport between subcellular compartments. Disease-causing mutations identified in each of the five complexes cause certain membrane proteins to be mislocalised.
Tumor-derived extracellular vesicles: molecular parcels that enable regulation of the immune response in cancer
Summary: This article reviews current literature on cargoes contained within extracellular vesicles (EVs) and the emerging theory of how they support immune suppression in cancer.
Cyclin-dependent kinase 1-mediated AMPK phosphorylation regulates chromosome alignment and mitotic progression
Summary: Multiple subunits of AMPK are phosphorylated by cyclin-dependent kinase 1 during mitosis. Phosphorylation of AMPK regulates proper mitosis and links to paclitaxel chemosensitivity.
VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1
Summary: VEGFR2 docked to shed Sdc1 activates CXCR4- and VLA-4-dependent tumor cell migration. Blocking VEGFR2 docking prevents this migration while stimulating the migration of tumor-targeting T cells and NK cells.
FKBP52 regulates TRPC3-dependent Ca2+ signals and the hypertrophic growth of cardiomyocyte cultures
Summary: The immunophilin FKBP52 directly interacts with TRPC3. A loss of interaction with the functionally intact FKBP52 results in an increased TRPC3 activity and enhanced enlargement of cardiomyocytes.
Mannose glycosylation is an integral step for NIS localization and function in human breast cancer cells
Highlighted Article: Insufficient expression of several enzymes involved in mannose glycosylation is responsible for defective NIS protein trafficking and localization in breast cancer cell.
The lncRNA hsrω regulates arginine dimethylation of human FUS to cause its proteasomal degradation in Drosophila
Summary: The lncRNA hsrω regulates the arginine methyltransferases type I and II to modify the human FUS RNA-binding protein, recombinantly expressed in flies, in a fashion that controls both its cellular localization and homeostasis.
Perturbed mitochondria–ER contacts in live neurons that model the amyloid pathology of Alzheimer's disease
Highlighted Article: Dynamic analysis of live neurons modelling Alzheimer's disease (AD)-like amyloid pathology shows loosened mitochondria-ER contacts (MERC), providing a novel perturbation of the MERC axis in AD.
Tox4 modulates cell fate reprogramming
Highlighted Article: Discovery of Tox4 as a mediator of reprogramming of mouse embryonic fibroblasts to the induced pluripotent and neuronal fate.
The Parkinson's disease gene PINK1 activates Akt via PINK1 kinase-dependent regulation of the phospholipid PI(3,4,5)P3
Summary: Loss of PINK1 kinase function, as occurs in Parkinson's disease, causes impaired activation of Akt as a result of defects in phospholipid PI(3,4,5)P3 regulation.
Inhibiting SUMO1-mediated SUMOylation induces autophagy-mediated cancer cell death and reduces tumour cell invasion via RAC1
Summary: Blockade of the SUMO pathway inhibits tumorigenesis through two different mechanisms: (i) stimulation of autophagy-mediated cancer cell death via TRIB3 upregulation; and (ii) inhibition of invasiveness via abrogation of RAC1 SUMOylation.
Identification of matrix physicochemical properties required for renal epithelial cell tubulogenesis by using synthetic hydrogels
Summary: A specifically engineered synthetic hydrogel platform that supports epithelial tubular morphogenetic programs was used to identify extracellular matrix biophysical and biochemical properties required for epithelial tubulogenesis.
Drosophila emerins control LINC complex localization and transcription to regulate myonuclear position
Highlighted Article: Two emerin proteins in Drosophila regulate the abundance of Klarsicht at the nucleus, which is necessary for the proper movement of myonuclei.
TGFβ-induced fibroblast activation requires persistent and targeted HDAC-mediated gene repression
Summary: TGFβ, a master regulator of fibrosis, epigenetically represses anti-fibrotic gene expression via HDACs to promote fibroblast activation.
An autism-associated mutation in GluN2B prevents NMDA receptor trafficking and interferes with dendrite growth
Highlighted Article: GRIN2B mutations cause ASD, but the pathogenic mechanisms remained unknown. We show that ASD-specific GRIN2B mutations interfere with NMDA receptor trafficking and lead to reduced dendrite elongation and branching.
Invadopodia-mediated ECM degradation is enhanced in the G1 phase of the cell cycle
Highlighted Article: Simultaneous time-lapse imaging of invadopodia and FUCCI markers in 2D, spheroids and tumors in vivo demonstrates that cancer cells invade preferentially during the G1 phase of the cell cycle.
Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis
Summary: Cytosolic annexin A5 regulates the particle binding step of clearance phagocytosis by increasing levels of apical surface αvβ5 integrin receptors of retinal pigment epithelial cells.
TOOLS AND RESOURCES
Heat resilience in embryonic zebrafish revealed using an in vivo stress granule reporter
Summary: Establishment of a new transgenic zebrafish line with knock-in GFP-G3BP1 to visualize stress granule dynamics in live animals in real time.
JCS Journal Meeting 2023: Imaging Cell Dynamics
Our 2023 Journal Meeting on ‘Imaging Cell Dynamics’ will be held from 14-17 May 2023 in Lisbon, Portugal. Due to popular demand, we can currently only accept applications for online attendance. Apply now to attend this meeting virtually. Registration deadline: 31 March.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
Editorial: Publishing where it matters
Editor-in-Chief Michael Way outlines Journal of Cell Science’s plans for the upcoming year and introduces Seema Grewal as our new Executive Editor.
preLights 5th Birthday webinar
preLights, our preprint highlighting service, is celebrating its 5th birthday this year. To mark the occasion, join us online on 14 March 2023 at 16:00 GMT for a discussion, led by four preLights alumni, on how to identify and navigate the challenges and opportunities while shaping your career as an early-career researcher.
Cell Scientists to Watch
As a community-focused journal, Journal of Cell Science is keen to support the next generation of cell biologists. Check out Cell Scientists to Watch, our interview series featuring talented researchers who have recently set up their own labs.