Issues
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Cover image
Cover Image
Cover: High frame rate three-dimensional views of the unicellular human parasite Trypanosoma brucei while swimming. Each image shows the same cell at different 3D orientations (left to right) at different times (top to bottom), where colour represents different focal planes (red to blue). See article by B. J. Walker and R. J. Wheeler (jcs231795).
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RESEARCH HIGHLIGHTS
STICKY WICKET
FIRST PERSON
MEETING REPORT
CELL SCIENCE AT A GLANCE
Intramembrane proteolysis at a glance: from signalling to protein degradation
Summary: In this Cell Science at a Glance article and the accompanying poster, we review the current knowledge on the function of intramembrane proteolysis in mammalian cells.
HYPOTHESIS
Dynamic cell–cell adhesion mediated by pericellular matrix interaction – a hypothesis
Summary: A discussion of the theory that pericellular matrices behave like hydrogels whose interactions can generate the entire 1000-fold range of observed cell–cell adhesion strengths.
REVIEW
Ubiquitin recognition in endocytic trafficking – with or without ESCRT-0
Summary: ESCRT-0 is a heterodimeric ubiquitin receptor involved in endosomal trafficking, that has been acquired in opisthokonts and missing in other organisms. We discuss how other multicellular organisms, such as plants, recognise ubiquitylated cargoes.
SHORT REPORT
High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella
Summary: A new 3D fluorescence microscopy-based method allows analysis of the high speed beating of cilia and flagella, with potential to answer open questions about Leishmania and Trypanosoma 3D motility.
RESEARCH ARTICLES
Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast
Highlighted Article: The regulatory domain of the COPI-associated ArfGAP Glo3 can stabilize the COPI coat. GTP hydrolysis is necessary to resolve the stabilised state. This mechanism is regulated by phosphorylation.
CXCL2 attenuates osteoblast differentiation by inhibiting the ERK1/2 signaling pathway
Highlighted Article: The inflammatory chemokine CXCL2 has an important role in the pathogenesis of osteoporosis and attenuates osteogenesis by inhibiting differentiation of osteoblasts through ERK1/2 signaling.
Serotonin receptor oligomerization regulates cAMP-based signaling
Highlighted Article: A FRET-based method is used to assess the impact of GPCR hetero-oligomerization on downstream signaling and contributes to the identification of the stoichiometry of endogenously expressed GPCRs under physiological conditions.
Trans-endocytosis elicited by nectins transfers cytoplasmic cargo, including infectious material, between cells
Highlighted Article: Characterization of a newly discovered nectin trans-endocytosis processes that not only transfers cell surface proteins but also cytoplasmic proteins. It can also transfer measles virus ribonucleocapsids, extending tropism to neurons.
Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria
Summary: Overexpression of the mitochondrial targeting sequence of ATP5G1 perturbs glucose metabolism, and translocates K-Ras to mitochondria and phosphatidylserine to endomembranes, through regulation of PI4P content at the Golgi.
Glucocorticoids delay RAF-induced senescence promoted by EGR1
Summary: In human fibroblasts, glucocorticoids induce a delay or bypass entry into senescence induced by oncogenic B-RAF-V600E. This senescence is promoted by EGR1 through transcriptional activation of the key cell cycle inhibitors CDKN1A and CDKN2B.
MKL1 overexpression predicts poor prognosis in patients with papillary thyroid cancer and promotes nodal metastasis
Summary: MKL1 expression is elevated in patients with papillary thyroid carcinoma (PTC) with nodal metastasis, promoting the migration and invasion of human PTC cells by targeting the TGF-β–Smad3–MMP2 signaling pathway.
Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons
Summary: The spine apparatus is assembled locally in dendrites. This process, which relies on the presence of synaptopodin and F-actin, is disrupted by interfering with myosin V activity.
The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface
Highlighted Article: The Chlamydomonas flagellar membrane glycoprotein FMG-1B is responsible for coupling the motor machinery of IFT to an extracellular substrate to generate flagellar surface motility (whole-cell gliding and microsphere movement).
PI3Kβ is selectively required for growth factor-stimulated macropinocytosis
Summary: There is a highly specific requirement for a single PI3K isoform, PI3Kβ, during growth factor-stimulated macropinocytosis and constitutive macropinocytosis in PTEN-null tumor cells.
Synergistic repression of thyroid hyperplasia by cyclin C and Pten
Summary: Cyclin C and the Pten phosphatase combine to restrict thyroid tissue hyperplasia by maintaining levels of the p21 and p53 tumor suppressors through a post-transcriptional mechanism.
The RNA-binding protein QKI controls alternative splicing in vascular cells, producing an effective model for therapy
Summary: The RNA-binding protein QKI isoforms hold key roles in alternative splicing facilitating the production of vascular cells for therapy and disease modelling.
PUBLISHER'S NOTE
Expression of Concern: Inhibition of TPO-induced MEK or mTOR activity induces opposite effects on the ploidy of human differentiating megakaryocytes (doi:10.1242/jcs.02784)
Interviews with Biologists @ 100 conference speakers

Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and Sadaf Farooqi, and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
The spatial choreography of mRNA biosynthesis

In their Review, André Ventura-Gomes and Maria Carmo-Fonseca detail the latest research progress and technological advancements that are helping to unlock how nuclear organisation underpins control of gene transcription and pre-mRNA splicing.
JCS-FocalPlane Training Grants

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction

In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.