CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Summary: This article highlights current progress on the role of the bone marrow microenvironment in health and disease.
Hematopoietic stem cells from induced pluripotent stem cells – considering the role of microRNA as a cell differentiation regulator
Summary: iPSC may be a new source for obtaining hematopoietic stem cells. Here, we discuss the challenges involved in reprogramming, and the role of microRNA in iPSC generation and HSC differentiation.
miRNA targeting and alternative splicing in the stress response – events hosted by membrane-less compartments
Summary: We review how the interplay between miRNA-based gene silencing of mRNAs and alternative splicing of pre-mRNAs are crucial post-transcriptional mechanisms that occur in liquid-like compartments to regulate cellular stress response.
Summary: Myo7a promotes tumor cell migration and metastasis in melanoma cells in a role that is distinct from the role it normally plays in specialized ciliated cells.
Highlighted Article: Septin proteins, which form cytoskeletal filaments and bind membranes, are required for efficient assembly of functional autophagic structures in budding yeast cells.
Highlighted Article: Tissues can sense external mechanical forces. By using a microfluidic compression device, we found that Ca2+ is an important messenger during the early phase of mechanical sensation, and regulates RhoA activity.
3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum
Highlighted Article: 3D correlative light and electron microscopy (3D-CLEM) reveals that replicative Brucella-containing vacuoles (rBCVs) are continuous with the ER, indicating that the pathogen replicates inside bona fide host ER.
Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis
Summary: Rapid condensin depletion reveals that different condensin levels are required for mitotic chromosome architecture and segregation. Condensin is not required for chromatin volume compaction during mitosis.
Summary: OSTF1 is a novel interaction partner for RP2, and this interaction is disrupted by a human pathogenic mutation in RP2. This provides a new link between RP2-associated pathogenesis and actin dynamics.
Summary: Inflachromene functions as an autophagy suppressor that inhibits HMGB1 nucleocytoplasmic translocation and enhances Beclin 1 degradation by interacting with RNF216 E3 Ub ligase, and could be a therapeutic factor for autophagy-related diseases.
Maturation of neural stem cells and integration into hippocampal circuits – a functional study in an in situ model of cerebral ischemia
Highlighted Article: Cerebral ischemia in situ impairs functional maturation of neurons derived from embryonic neural stem cells and boosts differentiation of embryonic stem cells into a glial cell type.
Summary: FGF2-dependent primary salivary mesenchyme and laminin-111 provide niche functions in salivary organoids to promote epithelial morphogenesis and expression of the early secretory proacinar markers Kit and AQP5.
Regulation of profibrotic responses by ADAM17 activation in high glucose requires its C-terminus and FAK
Summary: ADAM17 mediates fibrosis in diabetic kidney disease. The ADAM17 C-terminus and its phosphorylation at two sites are crucial for enzyme activation and the profibrotic response to high glucose.
Glycans modify mesenchymal stem cell differentiation to impact on the function of resulting osteoblasts
Summary: Both N- and O-glycan processing modulate MSC differentiation early during osteogenesis to influence mineral formation. Inhibition of N-glycan processing increases mineralization.