Summary: Image co-localization analysis is widely used but often misunderstood. This Review discusses important considerations, including applications to super-resolution microscopy.
Summary: This Review focuses on the role of lipid bilayer stress-induced UPR in ER homeostasis and discusses the sensing mechanism and its contribution to metabolic diseases.
Summary: This Review highlights the recent advance in identifying various factors that affect the aging process, focusing on how these determinants affect cell lifespan and the fitness of an organism.
Summary: A discussion of recent tomographic reconstructions that provide novel views on spindles and will enable us to revisit and address long-standing questions in the field of mitosis.
Yeast cells contain a heterogeneous population of peroxisomes that segregate asymmetrically during cell division
Summary: Yeast cells contain a mixture of young and old peroxisomes. During budding, the oldest ones are retained in the mother cell, whereas the younger organelles are transported to the bud.
The ARF guanine nucleotide exchange factor GBF1 is targeted to Golgi membranes through a PIP-binding domain
Summary: Our work represents the first report showing PIP binding by a large GEF GBF1 and the crucial importance of such binding in membrane recruitment.
Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function
Summary: An unbiased screen reveals the localization of ∼200 proteins in multiciliated cell. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.
Summary: Calcium signaling leads a morphogenetic process during neural rosette formation by regulating the movement of cell cytoskeletal elements.
Summary: A cell-based assay to probe the function of the ribbon structure of the Golgi unexpectedly demonstrated that the intact Golgi ribbon is essential for regulating the mTOR pathway and autophagy.
Summary: EFA6 proteins control epithelial luminogenesis by recruiting ACTN1 and regulating acto-myosin contractility. The EFA6–ACTN1 signaling pathway also restores an epithelial phenotype to mammary tumoral cells.
Nucleoplasmic lamins define growth-regulating functions of lamina-associated polypeptide 2α in progeria cells
Highlighted Article: Lamina-associated polypeptide (LAP) 2α promotes or inhibits proliferation of progeria cells depending on the absence or presence of lamin A in the nuclear interior.
The tail domain of the Aspergillus fumigatus class V myosin MyoE orchestrates septal localization and hyphal growth
Summary: We reveal the importance of the tail domain of the Aspergillus fumigatus class V myosin MyoE, and its role in growth and morphology.
Summary: Skeletal muscle nuclei sharing a common cytoplasm differ from one another in nuclear import. These differences in nuclear import could help establish regional specialization in large multinucleated muscle cells.
Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages
Summary: Abscisic acid, a natural hormone that confers plant tolerance to abiotic stresses, inhibits neovascularization by suppressing the angiogenic phenotypes of endothelial cells and macrophages, suggesting its potential usefulness in anti-angiogenic therapy.
MARCKS-related protein regulates cytoskeletal organization at cell–cell and cell–substrate contacts in epithelial cells
Highlighted Article: Deletion of MRP in epithelial cells resulted in dramatic changes in the organization of multiple actin-based structures, likely by regulation of integrin signaling.
Tight junctions negatively regulate mechanical forces applied to adherens junctions in vertebrate epithelial tissue
Summary: A FRET tension biosensor shows that tight junctions negatively regulate mechanical forces applied to adherens junctions. This regulation becomes essential when junctions are put under stress such as cytokinesis.
Blood vitronectin is a major activator of LIF and IL-6 in the brain through integrin–FAK and uPAR signaling
Summary: Blood vitronectin is a unique and key inducer of pro-inflammatory IL-6 and LIF, through integrin and uPAR signaling, which had not been previously indicated as having a role in cytokine expression.
Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion
Summary: Stress-activated MAPKs and the nuclear exportin CRM1 regulate translocation of the RhoA subfamily GEF Net1A from the nucleus to the cytoplasm to control RhoA signaling, cell motility and extracellular matrix invasion.
BMSCs ameliorate septic coagulopathy by suppressing inflammation in cecal ligation and puncture-induced sepsis
Summary: BMSCs effectively ameliorate septic coagulopathy, vascular damage, inflammation and acute lung injury, and improve the survival rate, suggesting that BMSC injection is a promising treatment for sepsis.
Rheb localized on the Golgi membrane activates lysosome-localized mTORC1 at the Golgi–lysosome contact site
Summary: Rheb is localized on the Golgi and activates mTORC1, which is localized on the lysosome. This activation occurs at an inter-organelle contact site termed the Golgi–lysosome contact site (GLCS).
TOOLS AND RESOURCES
Summary: A general strategy for conditional motor mutants that can be applied to kinesins and myosins inserts a protease cleavage site between the head and stalk domains, making it susceptible to specific proteolytic cleavage.