Unite to divide – how models and biological experimentation have come together to reveal mechanisms of cytokinesis
Summary: Biological experimentation and modeling have advanced our understanding of various cell behaviors. We provide a primer for biologists on several modeling strategies and highlight how modeling has revealed cytokinetic mechanisms.
Summary: Glycosphingolipids are emerging as powerful determinants in differentiation events. Here, we review the existing literature on the role of glycosphingolipids in cell fate determination.
Summary: Activation of Drosophila innate immune responses is coordinated at local and systemic levels through tissue crosstalk following disruptions to muscle homeostasis.
Summary: The small GTPase Rap1 binds directly to the F0 domain of Talin allowing its recruitment to integrins at the plasma membrane. This process appears to be strictly required for integrin-mediated adhesion in Drosophila.
Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia
Summary: Gli2 knockout in fibroblasts lengthens primary cilia through enhanced autophagy-mediated Ofd1 degradation. This ciliary lengthening delays cell cycle re-entry – an unexpected mechanism for cell cycle regulation.
ADAR2-mediated Q/R editing of GluK2 regulates kainate receptor upscaling in response to suppression of synaptic activity
Highlighted Article: Synaptic suppression promotes proteasomal degradation of the mRNA-editing enzyme ADAR2. Decreased ADAR2 levels reduce Q/R editing of the kainate receptor subunit GluK2 leading to enhanced surface expression and homeostatic upscaling of GluK2-containing KARs.
Lack of G1/S control destabilizes the yeast genome via replication stress-induced DSBs and illegitimate recombination
Summary: Absence of G1/S control in the budding yeast swi6Δ mutant leads to cell death due to replication stress and subsequent DSBs. Rad51 helps to repair breaks, but causes DNA rearrangements.
Summary: Here, we employ a novel technique to manipulate phosphatases, revealing a mechanism for how force transmission is switched off or reset.
Highlighted Article: An image-based screen using a dual labeling strategy identified bexarotene, a rexinoid, as a novel modulator of the primary cilium in VHL-deficient cells.
Highlighted Article: CEP97 degradation mediated by the CUL3–RBX1–KCTD10 E3 ubiquitin ligase is crucial for serum-starvation-induced CP110 removal and ciliogenesis.
An interaction network between the SNARE VAMP7 and Rab GTPases within a ciliary membrane-targeting complex
Summary: Two Rab proteins and a Rab GEF provide a ‘cradle’ for the longin SNARE VAMP7 mediating its incorporation into prebudding complex at the Golgi/TGN during sensory receptor ciliary trafficking.