Issues

Summary: In this Cell Science at a Glance article we summarize the current knowledge on the escape of viruses from the endosomal compartment and the cellular detection mechanisms that counteract it.

Summary: We summarize the molecular machinery shared between exosome biogenesis and the autophagy pathway, and describe the coordination between these two processes in multiple contexts, including neurodegeneration and cancer.

Summary: Long-term severe ethanol stress induces the formation of a unique high-ordered structure (multiple small cortical rings) of septin, accompanied by a depolarized actin cytoskeleton in yeast.

Summary: Integrin-dependent and integrin-independent mechanisms of glioma cell invasion in 3D brain-like environments indicate remarkable adhesion plasticity and escape routes from targeted intervention.

Highlighted Article: Cerebral cavernous malformation proteins control non-redundant ROCK1 and ROCK2 functions to preserve endothelial integrity. This study identified ROCK1 as a potential therapeutic target.

Summary: Rac1 activation promotes both the initial formation of AChR clusters as well as the dispersal of pre-existing clusters, whereas Rac1 inhibition is required to stabilize clusters.

Highlighted Article: Two different disease-associated FGFR2 mutations that lead to poor outcome in endometrial cancer patients, both affect collective cell polarity and invasion.

Summary: These data identify a new PIM-CHK1-PLK1 phosphorylation cascade that regulates different mitotic steps independently of the CHK1 DNA damage response function.

Summary: Connexin 43 in gap junctions becomes K63-polyubiquitylated on lysines 264 and 303, which is required for gap junction endocytosis.

Summary: The receptor PTPα is an essential component of integrin and dystroglycan receptor complexes that transduce extrinsic laminin-2 signals into specific intracellular events to promote OL differentiation.

Summary: Adenosine and its metabolism by adenosine kinase regulate autophagy independent of nutrient availability and the canonical AMPK–mTORC1 pathway. In β-cells, adenosine regulates insulin secretion, in part, through modulation of autophagy.

Highlighted Article: The enzyme PI4KIIIα works with two other proteins to produce PI4P, which feeds into the PIP₂ pool at the plasma membrane. This is required for PLC signaling during Drosophila phototransduction.

Summary: GRWD1 is a tumor-promoting factor. We comprehensively identified human GRWD1-interacting proteins, and performed functional analysis of GRWD1 during proteolytic regulation of RPL23, a tumor suppressor protein.

Highlighted Article: Myosin binding protein-C functionally interacts with the cardiac Ca²⁺ release channel (RyR2), suggesting a novel retrograde regulation mechanism for muscle excitation–contraction coupling.

Summary: The Cul4–DDB1–Gβ E3 ubiquitin ligase complex regulates Smoothened trafficking and Hedgehog signaling in Drosophila.

Summary: Development of a simple fluorescence imaging chamber allowing observation of intracellular protein dynamics and molecular events in living cells at pressure up to 200 bar.