IN THIS ISSUE
The resurrection of the PIDDosome – emerging roles in the DNA-damage response and centrosome surveillance
Summary: Discussion of the biology of the PIDDosome multiprotein complex and of recent advances that link PIDDosome-dependent CASP2 activation to p53 activation in response to extra centrosomes.
Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues
Highlighted Article: Neutrophils and macrophages display distinct modes of interstitial migration in vivo. While neutrophils are classic amoeboid cells, macrophages have a distinct morphology and use a more mesenchymal mode of migration in zebrafish larvae.
Summary: TFCP2L1 interacts with MTA1 to inhibit endoderm specification, while suppressing mesoderm and trophectoderm differentiation, in part, through downregulation of Lef1 expression.
Leucine-rich repeat-containing 8B protein is associated with the endoplasmic reticulum Ca2+ leak in HEK293 cells
Highlighted Article: LRRC8B participates in cellular Ca2+ homeostasis by modulating the endoplasmic reticulum Ca2+ leak.
The shape of the transmembrane domain is a novel endocytosis signal for single-spanning membrane proteins
Summary: A novel type of endocytosis signal for type II membrane proteins is encoded in the shape of their transmembrane domains.
The HECT E3 ubiquitin ligase NEDD4 interacts with and ubiquitylates SQSTM1 for inclusion body autophagy
Highlighted Article: NEDD4 is an autophagic HECT E3 ubiquitin ligase that ubiquitylates the autophagy receptor SQSTM1, facilitating inclusion body autophagy.
Summary: In addition to the canonical TRC40-targeting pathway, mammalian tail-anchored proteins can also utilise the SRP and SND pathways to facilitate their insertion into the ER membrane.
Interkinetic nuclear migration and basal tethering facilitates post-mitotic daughter separation in intestinal organoids
Highlighted Article: The long-term position of mitotic daughters in the intestinal epithelium correlates with their placement immediately after mitosis. An initial separation at birth, predicts more divergent positions later.
Summary: Pib2 and the EGO complex function in the same molecular pathway that leads to the activation of TORC1, a key regulator of cell growth.
Highlighted Article: This study describes a role for lipid ligand binding by a yeast ORP in exocytic vesicle docking, guiding our understanding of how ORPs may function in more complex organisms.
Constitutive activity of the Ghrelin receptor reduces surface expression of voltage-gated Ca2+ channels in a CaVβ-dependent manner
Summary: A new mechanism that modulates the trafficking of several CaV channel subtypes to the plasma membrane through the constitutive activity of the exceptional G-protein-coupled receptor GHSR.
A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis in Trypanosoma brucei
Summary: Analysis of TOEFAZ1, an essential kinetoplastid-specific cytokinetic protein, identifies the function of individual domains and implicates the protein in the positioning and timing of T. brucei cleavage furrow ingression.
TOOLS AND RESOURCES
Summary: The γ2pHFAP and MG dye paired optical reporter system is an adaptable fluorescent tool that can be used to simultaneously measure multi-stage GABAAR trafficking in real-time in living neurons.