Issues
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Cover image
Cover Image
Cover: Fluorescence micrograph of a human embryonic retinoblast (HER)-911 cell that has been inoculated with Atto565-tagged adenovirus type 2 (Ad2), treated with leptomycin B (LMB) to block detachment of virions from microtubules, and fixed and stained with anti-β-tubulin antibodies. Images were acquired by 3D time-gated stimulated emission depletion microscopy (3D-gSTED). Note that LMB leads to virion enrichment in the center of the cell (large red dot), the microtubule-organizing center (not shown). Maximal projections of Z-stacks show Atto565-tagged virions (red) and microtubules stained with antibody against β-tubulin (cyan). Note that the brightness of the cyan signal correlates with the z-position of the optical section; microtubules on top of the cell appear in bright cyan and those at the bottom of the cell in dark blue. Image provided by I.-H. Wang. See article by I-H. Wang et al. (pp. 2185–2195).
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IN THIS ISSUE
STICKY WICKET
CELL SCIENTISTS TO WATCH
MEETING REPORT
COMMENTARIES
Lamins in the nuclear interior − life outside the lamina
Summary: We discuss recent advances in the regulation of the lamina-independent pool of lamins in the nucleoplasm and their emerging functions in chromatin organization, mechanical regulation and disease.
Molecular mechanisms of kinesin-14 motors in spindle assembly and chromosome segregation
Summary: During cell division, kinesin-14 motors are conserved regulators of spindle assembly and chromosome segregation. We provide a detailed view of their cellular functions in diverse organisms.
SHORT REPORT
Substrate stiffness-dependent regulation of the SRF−Mkl1 co-activator complex requires the inner nuclear membrane protein Emerin
Summary: The nuclear lamina regulates SRF−Mkl1 activity specifically in cells grown within stiff mechanical environments.
RESEARCH ARTICLES
Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis
Highlighted Article: Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics and trafficking of plasma membrane receptors through clathrin-mediated endocytosis in Drosophila cells.
Crosstalk from cAMP to ERK1/2 emerges during postnatal maturation of nociceptive neurons and is maintained during aging
Summary: Differentiation of nociceptors relies on transcription factors, receptors and neuropeptides. We identify the importance of intracellular signaling, including pathways involved in neuronal memory, for postnatal nociceptor maturation.
Unique cell biological profiles of retinal disease-causing missense mutations in the polarity protein Crumbs
Highlighted Article: Analysis of Drosophila crb mutations demonstrates that the Crb extracellular region controls protein level and distribution, and suggests that human disease-causing CRB1 missense mutations affect rhodopsin trafficking.
A Wntless–SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export
Summary: WLS and SEC12 form a stable complex on ER membrane that binds to mature Wnt molecules. We find that several independent protein motifs within WLS are essential for the association of this complex with COPII ER-exiting machinery.
A dual phenotype of MDA-MB-468 cancer cells reveals mutual regulation of tensin3 and adhesion plasticity
Highlighted Article: The cell−matrix adapter protein Tns3 is identified as a determinant of adhesion plasticity in subcultures of cancer cells. Tns3 gene expression constitutes a feedback loop controling cell adhesion and motility.
The nuclear export factor CRM1 controls juxta-nuclear microtubule-dependent virus transport
Summary: The mechanism through which macromolecular complexes reach the nuclear pores is incompletely understood. We show that the cytoplasmic perinuclear ecology is tuned by CRM1 to control virus transport to the nuclear pore complex.
TRPV4 mediates the Ca2+ influx required for the interaction between flightless-1 and non-muscle myosin, and collagen remodeling
Highlighted Article: TRPV4 is a Ca2+-permeable channel in the plasma membrane that regulates FliI–NMMIIA interactions, which in turn affects the cell extension formation necessary for the control of collagen remodeling and matrix biology.
The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to the axon initial segment in hippocampal neurons
Summary: The selective distribution of the cell adhesion molecules Caspr2 and TAG-1 might be responsible for positioning of Kv1 channels at the axon initial segment versus along the axon in hippocampal neurons.
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