IN THIS ISSUE
CELL SCIENTISTS TO WATCH
Summary: Microbial pathogens have developed a variety of strategies, both conventional and unconventional, to exploit and interfere with the ubiquitylation machinery of infected host cells.
Summary: This Commentary discusses poorly appreciated paradigms of how DUBs are activated, regulated and targeted to their appropriate substrates to achieve precise spatio-temporal control of ubiquitin signals within a specific biological pathway.
Summary: Rab5 recruits the Vps34-containing PI3K to regulate endosome trafficking. We identified a negative feedback loop whereby C. elegans VPS-34 recruits the TBC-2 RAB-5 GAP to endosomes to inactivate RAB-5 during endosome maturation.
In vivo reprogramming of non-mammary cells to an epithelial cell fate is independent of amphiregulin signaling
Highlighted Article: The estrogen-regulated growth factor amphiregulin, which is necessary for post-pubertal mammary gland ductal elongation, is not required for reprogramming non-mammary stem cells to a mammary cell fate.
Summary: Bone erosion requires that osteoclasts both resorb and migrate. According to common belief, these activities are mutually exclusive and alternate. Paradoxically, we show here simultaneous resorption and migration.
ADAM12 induction by Twist1 promotes tumor invasion and metastasis via regulation of invadopodia and focal adhesions
Highlighted Article: Induction of ADAM12 by Twist1 promotes invadopodia formation and focal adhesion turnover to facilitate Twist1-induced cell migration, invasion and tumor metastasis.
Summary: Using a novel system to identify cell growth regulators, we have discovered a number of factors that activate growth in cells in which growth is developmentally switched off.
Highlighted Article: We show that Kif5b can compensate for defects in myosin-Va-based transport in mammals, but that endogenous Kif5b plays a minimal role in transport in melanocytes due to inefficient recruitment to melanosomes.
TOOLS AND TECHNIQUES
Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data
Summary: We combined super-resolution microscopy with robust image analyses to discern the organization of chromatin at nuclear pore complexes. This demonstrated the importance of image analysis to enhance super-resolution techniques.