IN THIS ISSUE
CELL SCIENTISTS TO WATCH
Summary: Overview of the multiple cellular and physiological functions of Arl1 at the TGN, focusing on the associated regulators and effectors, and the pathways they control.
Summary: Citron kinase has been recently found to have new functions throughout the cell cycle and be involved in human disease. These findings and future perspectives are discussed in this Commentary.
CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin
Summary: Non-centrosomal microtubules are nucleated at centrosomes and then released; here, we show that CAMSAP3 coordinates with dynein and katanin to mediate this release.
Highlighted Article: The nuclear α-catenin–β-catenin complex mediates DNA damage sensitivity in a WNT-dependent manner. α-Catenin recruitment to sites of DNA damage is dependent on β-catenin and nuclear actin.
Summary: The γ isoform of the formin FMNL1 localizes to podosomes and is required for efficient macrophage migration, independent of its FH2 actin-binding domain.
Unphosphorylated STAT1 represses apoptosis in macrophages during Mycobacterium tuberculosis infection
Highlighted Article: Accumulated unphosphorylated STAT1 decreases apoptosis of macrophages by interacting with STAT3 to repress expression of CD95 at the later stages of Mycobacterium tuberculosis infection.
Spatial distribution and molecular dynamics of dystrophin glycoprotein components at the neuromuscular junction in vivo
Summary: Utrophin is a key component bridging rapsyn and AChRs to α-dystrobrevin and α-syntrophin in the AChR-rich domain, and we show that α-syntrophin and rapsyn turn over more rapidly than AChRs at the NMJ.
Highlighted Article: Cdc20-dependent activation of the APC/C is essential for chromosome segregation and exit from mitosis. We show that the PP2AB56 phosphatase is required for the assembly of APC/CCdc20.
Summary: Adhesion receptor integrin-α3 is involved in presynaptic differentiation, synaptic maintenance and nerve terminal anchorage at the neuromuscular synapse.
Highlighted Article: An unusual FACS-based screen using Dictyostelium identifies Mroh1 – a conserved protein with HEAT repeats – as a WASH and F-actin target that is important for constitutive exocytosis of lysosomes.
Summary: Identification of a novel structure related to the nucleoplasmic reticulum in polyploid and stressed cells. Active mRNA translation occurs in these structures, helping polyploid cells to maintain protein synthesis.
Highlighted Article: This article demonstrates that Centralspindlin is tethered to microtubule plus ends via an SxIP motif, and disruption of this motif causes defects in cytokinesis and cell–cell junctions.
Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
Summary: There are compositional and structural differences in the nuclear pore complexes present in the functionally differentiated macronucleus and micronucleus within the single cytoplasm of ciliated protozoa.
Highlighted Article: Spermatid protein kinase TSSK6 is essential for fertility. Here, we show that TSSK6 is necessary for H2AX phosphorylation at Ser139 and histone-to-protamine transition during spermiogenesis, and link γH2AX to normal sperm development.
TOOLS AND TECHNIQUES
Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis
Summary: Spatial analysis of microtubule organisation in mitotic cells by using 3D imaging and mathematical modelling.