Issues

Summary: A review of the current knowledge of the timeline of clathrin-mediated endocytosis in the budding yeast S. cerevisiae, including discussion of recent works that focus on how this process is regulated.

Summary: This Commentary discusses how partitioning of membrane proteins can affect their trafficking between the ER and the Golgi in the forward and backward directions.

Summary: Axonemal outer dynein arms can form 24-nm repeats by themselves without docking complexes. The docking complex is likely to be a flexible scaffold rather than a molecular ruler.

Highlighted Article: TMEM170A is a human ER and nuclear envelope transmembrane protein. Down- and overexpression induce tubular or sheet ER formation, respectively, indicating that TMEM170A is a newly discovered ER-sheet-promoting protein.

Summary: NOL1 is a TERC-binding protein and represents a new method by which telomerase activates transcription of the cyclin D1 gene, thus maintaining cell proliferation capacity.

Highlighted Article: This manuscript details the mechanistic pathways linking oxLDL and NF-κB activation, and shows for the first time that endothelial FAK activity contributes to atherosclerosis.

Summary: Maturation of mitotic pre-rRNAs is transiently arrested during mitosis by a CDK1–cyclin-B-dependent mechanism. Strikingly, during their maturation process, mitotic pre-rRNAs localize in reforming nucleoli.

Highlighted Article: The importance of localised regulation of protein dephosphorylation in cell adhesion and migration through demonstration of a new interaction between the LIM protein LPP and PP2A comprising the Bʹʹ/PR130 subunit.

Summary: Conditional mutation of Smc5 in mouse embryonic stem cells leads to obstruction of chromosome condensation, congression and segregation, accompanied by abnormal spindles, lagging chromosomes and DNA bridges.

Highlighted Article: The ER within Drosophila motor nerve terminals forms a network of tubules. Loss of the fusion GTPase atlastin fragments synaptic ER. Alteration of ER structure decreases evoked neurotransmitter release.

Summary: Sustaining patterns of gene expression through the yeast CWI pathway are mainly achieved by positive Rlm1 autoregulatory circuits, which are essential for amplification of the transcriptional response.

Highlighted Article: HSPB7 plays a crucial role in the maintenance of the muscle integrity, possibly through stabilizing the function of FLNC.

Summary: A new association between hereditary myopathy and CLOCK circuit genes opens novel avenues of research on circadian genes and their role in skeletal muscle hereditary pathologies.

Summary: Myosin 1b and 1c localize to fused secretory vesicles and influence the compression of the actin coat during exocytosis.

Summary: Binding of influenza A virus ribonucleoproteins to the recycling endosome modulates its function, decreasing its sorting efficiency and inducing vesicular clustering.

Summary: Regulatory endocytosis and recycling processes of CTR1 via clathrin-mediated and Rab11 pathways enable cells to dynamically alter transporter levels at the plasma membrane and acutely modulate Cu entry.

Summary: GtaG is involved in the production of two culmination-signaling molecules – c-di-GMP and the spore differentiation factor SDF-1. Addition of c-di-GMP rescues culmination and spore-formation deficiencies of gtaG mutant.

Summary: Rab8 can induce Rac1- and Tiam1-dependent cortical actin polymerization and focal adhesion disassembly through the proteases MT1-MMP and calpain, and Rho-GTPase-dependent mechanisms.