IN THIS ISSUE
CELL SCIENCE AT A GLANCE
Summary: Assembly of actin filaments at barbed ends drives motile processes. Here, we discuss the control of polarized filament assembly by various regulators that interact with and compete for barbed ends.
Summary: Multiple layers of crosstalk between integrins, cadherins and forces drive spatial organization of signaling and forces within cells.
SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration in 1321 N1 glioblastoma cells
Highlighted Article: SHIP2 modulates the balance between PI(4,5)P2 and PI4P, thereby participating in the control of migration in 1321 N1 glioblastoma cells through the organization of focal adhesions.
Summary: NTF2 reduces the diameter of the NPC, nuclear import of large cargos and nuclear size in a Ran-binding-dependent manner, in both Xenopus and mammalian cells.
Correctors of mutant CFTR enhance subcortical cAMP–PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization
Highlighted Article: Correctors of F508del CFTR, by activating ezrin and actin cytoskeleton re-organization, generate a sub-cortical pool of cAMP of adequate amplitude to activate chloride secretion.
Summary: Post-transcriptional regulation of NMHC IIA protein expression by hnRNP K contributes to the process of enucleation in induced erythroid maturation.
Highlighted Article: A tetraspanin family protein, Tsp2A is an essential component of septate junctions in the Drosophila endodermal epithelia and is involved in intestinal barrier function.
Inhibition of the PI3K–Akt and mTORC1 signaling pathways promotes the elongation of vascular endothelial cells
Summary: PI3K–Akt and mTORC1 inhibitors induce angiogenic elongation of vascular endothelial cells without an excessive VEGF stimulation.
Proteasome storage granules are transiently associated with the insoluble protein deposit in Saccharomyces cerevisiae
Summary: Carbon source depletion in Saccharomyces cerevisiae leads to the triage of proteasome storage granules (PSGs) and dysfunctional proteasomes on the IPOD, to ensure that only structurally ‘correct’ proteasomes are deployed.
Soluble LILRA3 promotes neurite outgrowth and synapses formation through a high-affinity interaction with Nogo 66
Summary: LILRA3 protein is a soluble new high-affinity ligand to Nogo 66 and promotes neurite outgrowth and synapse formation in primary cortical neurons by antagonising Nogo-66-mediated inhibition.
Activator of G-protein signaling 8 is involved in VEGF-mediated signal processing during angiogenesis
Summary: Activator of G-protein signaling 8 (AGS8) regulates the angiogenic events in endothelial cells by influencing the intracellular distribution of VEGFR-2. A new signal input to angiogenesis by AGS8 is suggested.
Summary: Monoubiquitylation (mUb) of PCNA is a crucial event coordinating DNA damage tolerance pathways. We report here that translesion synthesis polymerase REV1 can promote PCNA–mUb through its preferential association with RAD18–mUb.
Summary: Pak1 and its downstream effector cofilin play an essential role in VEGFA-induced angiogenic events in human retinal microvascular endothelial cells in vitro and hypoxia-induced retinal neovascularization in vivo.
Summary: Identification of a new fatty acid synthase inhibitor for nuclear division by a chemical genetic screen revealed a link between nuclear envelope expansion and faithful chromosome segregation in a closed mitosis.
TOOLS AND TECHNIQUES
Highlighted Article: Disequilibrium between aggregate formation and clearance leads to accumulation of aggregated proteins. We have created a new cell-based assay that can determine how modifiers can impact on aggregate burden.
Highlighted Article: The RNA-binding proteins DAZL and CPEB1 cooperate to regulate mRNA translation and protein synthesis during the meiotic cell cycle in mouse oocytes.