IN THIS ISSUE
CELL SCIENCE AT A GLANCE
Summary: This article indicates metabolic pathways essential for cancer growth and survival, with an emphasis on anticancer therapies that are associated with such metabolic vulnerabilities.
Summary: In development and wound repair, cellular unjamming promotes cooperative cellular migration, whereas in cancer invasion, asthma and aberrant wound repair, it unleashes remarkably asocial cellular misbehavior.
Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis
Highlighted Article: Mycobacterial infection in zebrafish larvae was explored using a combination of light and electron microscopy. Macrophage burst events resulted in a progression of infection, whereas epithelium-mediated extrusion of infected immune cells abated infection.
The RNF146 and tankyrase pathway maintains the junctional Crumbs complex through regulation of angiomotin
Summary: We describe a new role for RNF146 and tankyrase in maintaining the Crumbs complex and apical-basal integrity in epithelial cells through regulation of ADP-ribosylation and ubiquitin-dependent degradation of the angiomotin AmotL2.
Highlighted Article: Depletion of actin in the nucleus by polymerization into stable nuclear actin filaments inhibits the actin–polymerase-II interaction, resulting in impaired RNA polymerase II activity, localization, and gene recruitment.
Drosophila Kette coordinates myoblast junction dissolution and the ratio of Scar-to-WASp during myoblast fusion
Summary: The Drosophila protein Kette is essential for myoblast fusion. It controls the dissolution of electron-dense plaques and the ratio of Scar and WASp proteins in fusion-competent myoblasts during fusion pore formation.
Highlighted Article: Bradykinin induces neuron-generating division in vitro and in vivo, lengthening the G1 phase of the cell cycle and TIS21 expression. Neurogenesis through kinin-B2 receptor signaling was dependent on sustained ERK activation.
Summary: Tensin 3 regulates podosome patterning in osteoclasts and bone resorption. Here, we show that tensin 3 is a partner of the Rac exchange factor Dock5 that stimulates its activity.
Local Ras activation, PTEN pattern, and global actin flow in the chemotactic responses of oversized cells
Highlighted Article: The chemotactic response system of Dictyostelium cells can accommodate larger cells, in a way that involves asymmetric Ras activation in turning protrusions, unconventional PTEN patterns and tail-directed actin flow during the establishment of polarity.
Proplatelet formation is selectively inhibited by collagen type I through Syk-independent GPVI signaling
Summary: Binding of collagen type I to GPVI on megakaryocytes inhibits premature proplatelet formation by a Syk-independent pathway that is distinct from GPVI signaling in platelets.
Summary: TGF-β2 promotes K+-Cl−-co-transporter KCC2 trafficking and functional activation in hippocampal neurons through a TGF-β2, CREB and Rab11b signaling pathway.
Summary: Axonal transport of kinesin-1 and microtubule dynamics are differentially regulated between terminals in a single axon, and contribute to shaping the axonal arbor by differentially regulating retraction of terminals.
TOOLS AND TECHNIQUES
Visualizing red blood cell sickling and the effects of inhibition of sphingosine kinase 1 using soft X-ray tomography
Highlighted Article: Soft X-ray cryotomography revealed an inverse relationship between sickle RBC volume and number of protrusions upon sickling. Subtomogram analysis showed beneficial effects with Sphk1 inhibitor treatment.