IN THIS ISSUE
CELL SCIENTISTS TO WATCH
CELL SCIENCE AT A GLANCE
Summary: Integrin-dependent and -independent function of kindlin isoforms and their regulation in homeostasis, disease and cancer.
The NF-κB-modulated microRNAs miR-195 and miR-497 inhibit myoblast proliferation by targeting Igf1r, Insr and cyclin genes
Summary: miR-195 and miR-497 are negatively regulated by the myogenic factor NF-κB, and they could inhibit myoblast proliferation through targeting the Igf1r, Insr and cyclin genes.
LPS impairs oxygen utilization in epithelia by triggering degradation of the mitochondrial enzyme Alcat1
Highlighted Article: This study provides new insight into how endotoxin triggers depletion of a key mitochondrial enzyme, Alcat1, by activating an SCF ubiquitin E3 ligase complex that contains Fbxo28.
Activated microglia cause reversible apoptosis of pheochromocytoma cells, inducing their cell death by phagocytosis
Summary: NO from microglia causes reversible caspase activation and phosphatidylserine exposure by PC12 cells, resulting in their cell death by phagocytosis.
Highlighted Article: The distinct activities of p120 towards E-cadherin and Rho are molecularly and functionally coupled, and this in turn enables the maintenance of cell shape in the larger context of an epithelial monolayer.
Summary: Palmitoylation and proximal sequences can function as secondary sorting signals for membrane proteins with suboptimal transmembrane domains.
Evolutionarily conserved primary TNF sequences relate to its primitive functions in cell death induction
Summary: By using a series of overlapping human TNF-derived peptides, it is shown that the evolutionarily conserved primary sequences of TNF relate to its primitive role in cell death.
Summary: Detailed analysis of the ability of the Drosophila cytolinker protein Pigs to modulate its cytoskeleton-interacting functions (+TIP tracking, microtubule shaft binding, actin binding and actin–microtubule crosslinking).
Summary: Screening of known kinases in the yeast genome revealed the Ccl1–Kin28 complex as a novel regulator of autophagy. Proteasomal turnover of Ccl1 limits autophagy during prolonged starvation.
Summary: This study demonstrates the reciprocal roles of the transcriptional repressor Gfi1b and its gene target and signaling factor Rgs18 in arbitrating the generation of the erythroid and megakaryocytic lineages.
Summary: NAF-1 is a major player in the metabolic regulation of breast cancer cells through its effects on cellular Fe ion distribution, mitochondrial metabolism and the induction of apoptosis.
BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission
Summary: A loss-of-function study of the SNX9, SNX18 and SNX33 homolog SH3PX1 in Drosophila reveals a new role in the development of presynaptic active zones and regulation of neurotransmitter release.
Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons
Summary: Novel imaging procedures in the adult Drosophila wing reveal that Lissencephaly-1 restrains mitochondrial motion and that reducing levels of this protein protects against an age-related decline in protein homeostasis.
Summary: CDK-mediated phosphorylation of PHD1 at serine 130 controls target specificity and confers cell cycle regulation of PHD1.
Summary: We reveal the molecular mechanism by which BMP6 induces hyperpermeabilization of the endothelium. This provides first insights into the mechanism of BMP-dependent vascular integrity in normal physiology and disease.
The murine cytomegalovirus immunoevasin gp40 binds MHC class I molecules to retain them in the early secretory pathway
Summary: The murine cytomegalovirus immunoevasin gp40 effectively reduces MHC class I surface levels by binding to them and retaining them in the early secretory pathway.
TOOLS AND TECHNIQUES
Localisation-based imaging of malarial antigens during erythrocyte entry reaffirms a role for AMA1 but not MTRAP in invasion
Highlighted Article: Here we develop a high-definition imaging approach to dissect and assign function to proteins involved in the rapid process of malaria parasite invasion of the human erythrocyte.