Issues
-
Cover image
Cover Image
Cover: MCF10A breast epithelial cells expressing HDAC4–GFP (green) grown in 3D culture for 8 days and stained with anti-GM130 to visualize the Golgi (red) and with TOPRO-3 to identify nuclei (blue). Confocal microscopy section of the equatorial plane. The MEF2–HDAC axis influences epithelial cell proliferation. See article by A. Clocchiatti et al. (pp. 3961–3976).
- PDF Icon PDF LinkTable of contents
- PDF Icon PDF LinkIssue info
IN THIS ISSUE
STICKY WICKET
CELL SCIENTISTS TO WATCH
MEETING REPORT
OPINION
Reliability of ROS and RNS detection in hematopoietic stem cells − potential issues with probes and target cell population
Summary: In this Opinion article we discuss the reliability of ROS and RNS detection in HSCs, and propose approaches to examine the levels of ROS and RNS specifically in HSCs in vitro and in vivo.
COMMENTARY
Surviving protein quality control catastrophes – from cells to organisms
Summary: The limits of the cellular resilience to protein misfolding are outlined, and the possibility of pushing these limits to help cells survive and to avoid protein quality control catastrophes is discussed.
SHORT REPORT
LPP3 localizes LPA6 signalling to non-contact sites in endothelial cells
Summary: In endothelial cells, the membrane-bound LPA-degrading enzyme LPP3 is specifically expressed at cell–cell contact sites, thereby localizing the signal evoked by extracellularly produced LPA.
RESEARCH ARTICLES
Galactosylsphingosine (psychosine)-induced demyelination is attenuated by sphingosine 1-phosphate signalling
Highlighted Article: We provide data suggesting that sphingosine 1-phosphate signalling could be a potential drug target for Krabbe disease and perhaps other demyelinating illnesses, in addition to multiple sclerosis.
Two-color fluorescent analysis of connexin 36 turnover: relationship to functional plasticity
Summary: Fluorescent labeling of Cx36-HaloTag revealed that turnover occurs on a different time scale than phosphorylation-driven plasticity. Cx36-HaloTag is also partially mobile within gap junctions.
EGF augments TGFβ-induced epithelial–mesenchymal transition by promoting SHP2 binding to GAB1
Highlighted Article: By activating ERK1/2, the protein tyrosine phosphatase SHP2 tunes the extent of the epithelial–mesenchymal transition observed in carcinoma cells in response to different growth factors.
Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2
Summary: The yeast GSK-3 kinase controls astral microtubule functions by regulating the interaction of the microtubule-stabilising kinesin Kip2 with microtubules and its processivity factor Bim1/EB1.
The ARF2–ANT–COR15A gene cascade regulates ABA-signaling-mediated resistance of large seeds to drought in Arabidopsis
Highlighted Article: This work reveals, at a molecular level, that seedlings of large-seeded plants can withstand drought stresses efficiently because of ABA signaling mediating a ARF2–ANT–COR15A gene cascade.
Rac1 augments Wnt signaling by stimulating β-catenin–lymphoid enhancer factor-1 complex assembly independent of β-catenin nuclear import
Summary: Evidence is presented for a novel role of Rac1 in stimulating β-catenin–LEF-1 complex formation.
Cx30 exhibits unique characteristics including a long half-life when assembled into gap junctions
Highlighted Article: The gap junction protein Cx30 was found to be an unusually stable, long-lived connexin, which might underlie its specific role in the epidermis and cochlea.
The MEF2–HDAC axis controls proliferation of mammary epithelial cells and acini formation in vitro
Summary: The HER2 oncogene alters the epithelial morphogenetic program and inhibits MEF2 transcriptional programs. Reactivation of MEF2s re-established normal acini morphogenesis.
Eukaryotic initiation factor 6 modulates myofibroblast differentiation at transforming growth factor-β1 transcription level via H2A.Z occupancy and Sp1 recruitment
Highlighted Article: Eukaryotic initiation factor 6 (eIF6) acts to facilitate Sp1 recruitment to the TGF-β1 promoter through H2A.Z depletion and thus results in increased TGF-β1 transcription, which contributes to myofibroblast differentiation.
Altered N-glycosylation modulates TgrB1- and TgrC1-mediated development but not allorecognition in Dictyostelium
Summary: In Dictyostelium, compromised N-glycosylation, but not ER stress, caused by tunicamycin treatment or alg9 mutation, partially suppresses the developmental arrest in tgrB1-tgrC1-defective mutants.
Focal-adhesion-independent integrin-αv regulation of FAK and c-Myc is necessary for 3D skin formation and tumor invasion
Summary: Integrin-αv plays a crucial role in regulating skin tissue generation and tumor invasion through a focal-adhesion-independent FAK–p38–p90RSK–c-Myc signaling axis.
Uncoupling PIP2-calmodulin regulation of Kv7.2 channels by an assembly destabilizing epileptogenic mutation
Summary: Conformational changes in the helix D coiled-coil of the Kv7.2 channel affect interactions with calmodulin, which binds to helices AB, modulating channel gating and PIP2 sensitivity.
CORRECTIONS
ARTICLES OF INTEREST IN OTHER COB JOURNALS
From Development
Call for papers - Cilia and Flagella: from Basic Biology to Disease
We are welcoming submissions for our upcoming special issue: Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Submission deadline: 1 March 2025.
Biologists @ 100 - join us in Liverpool in March 2025
We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK.
Principles and regulation of mechanosensing
Mechanics play a fundamental role in cell physiology and represent physical mechanisms which cells use to influence function from the molecular to tissue scale. In this Review, Stefano Sala and colleagues clearly define mechanosensing and mechanotransduction, illustrate various mechanosensing mechanisms and discuss methods that cells use to regulate these processes.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2024 applications: 7 September (decision by week commencing 8 October 2024); 22 November (decision by week commencing 16 December).
