Issues
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Cover image
Cover Image
Cover: In the visible immunoprecipitation (VIP) assay, protein-protein interactions can be visualized by directly observing immunoprecipitated fluorescent fusion proteins under a fluorescence microscope without SDS-PAGE and immunoblotting. This image shows a mixture of immunoprecipitated beads decorated with various fluorescent proteins after VIP assays. See article by Y. Katoh et al. (pp. 2351–2362).
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IN THIS ISSUE
CELL SCIENCE AT A GLANCE
The contractome – a systems view of actomyosin contractility in non-muscle cells
Summary: A systematic analysis of the non-muscle myosin II literature reveals the multitude of cellular processes in which contractility plays a role and the complex protein interaction network regulating it.
COMMENTARY
SEA you later alli-GATOR – a dynamic regulator of the TORC1 stress response pathway
Summary: The multiprotein SEA/GATOR complex is a major upstream regulator of TORC1. This Commentary describes its structural and functional features, and provides an overview of the consequences of its deregulation in cancer.
SHORT REPORT
The juxtamembrane region of synaptotagmin 1 interacts with dynamin 1 and regulates vesicle fission during compensatory endocytosis in endocrine cells
Highlighted Article: An interaction between the juxtamembrane domain of synaptotagmin 1 and the pleckstrin homology domain of dynamin 1 regulates endocytic fission in chromaffin cells.
RESEARCH ARTICLES
Autocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy
Highlighted Article: Intracellular VEGF signaling in endothelial cells regulates mitochondria function and levels of FOXO1.
JNK2 controls fragmentation of the Golgi complex and the G2/M transition through phosphorylation of GRASP65
Summary: JNK2-mediated phosphorylation of GRASP65 at Ser277 has a crucial role in the G2-specific separation of the Golgi stacks that is required for the G2/M transition.
The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
Summary: MAL, an integral membrane protein component of the apical transport machinery, regulates the condensation of membranes at the ciliary base for efficient primary ciliogenesis in epithelial MDCK cells.
Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells
Highlighted Article: The epithelial cholinergic system activates a signaling pathway that regulates paracellular permeability by modulating the content and distribution of the tight junction protein claudin-4.
Akt-mediated phosphorylation increases the binding affinity of hTERT for importin α to promote nuclear translocation
Summary: Akt-mediated phosphorylation of residue S227 within the nuclear localization signal of hTERT increases the binding affinity for importin α and promotes nuclear import of hTERT.
GABAB receptor promotes its own surface expression by recruiting a Rap1-dependent signaling cascade
Summary: GABAB receptor activation induces Rap1 activation to promote its own surface expression by stimulating receptor recycling, thereby providing a potential explanation for the long-lasting action of GABA.
Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males
Highlighted Article: miRNA-deficient spermatocytes display frequent sex chromosome fusions and fail to progress through meiosis in a process that is probably mediated by dysregulation of Atm.
Control of Wnt5b secretion by Wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression
Summary: Wntless-mediated Wnt5b secretion modulates the proliferation of chondrogenic cells through fine-tuning regional Fgf3 expression during the development of craniofacial cartilages.
Mecp2 regulates neural cell differentiation by suppressing the Id1 to Her2 axis in zebrafish
Summary: Mecp2 suppresses neural cell development by suppressing the Id1–Her2 (Hes5 in mammals) axis, which is a mechanism that might contribute to the later motor and cognitive dysfunctions in Rett syndrome.
Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins
Highlighted Article: The architectures of the BBSome and exocyst are revealed by a visible immunoprecipitation assay, which can detect interactions between fluorescent fusion proteins without performing western blotting.
Rab32 is essential for maintaining functional acidocalcisomes, and for growth and infectivity of Trypanosoma cruzi
Summary: In addition to its role in osmoregulation, the contractile vacuole of Trypanosoma cruzi, the agent of Chagas disease, has a role in the biogenesis of acidocalcisomes.
Sonic hedgehog processing and release are regulated by glypican heparan sulfate proteoglycans
Summary: Cell surface heparan sulfate proteoglycans act as platforms for hedgehog morphogen assembly and storage on producing cells, but also act as a scaffold for factors involved in their regulated release.
ARTICLES OF INTEREST IN OTHER COB JOURNALS
From Development
Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front
Call for papers - Cilia and Flagella: from Basic Biology to Disease
We are welcoming submissions for our upcoming special issue: Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Extended submission deadline: 31 March 2025.
History of our journals
As our publisher, The Company of Biologists, turns 100 years old, read about Journal of Cell Science’s journey and explore the history of each of our sister journals: Development, Journal of Experimental Biology, Disease Models & Mechanisms and Biology Open.
Introducing our new Associate Editors
In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
Diversity of microtubule arrays in animal cells at a glance
In this Cell Science at a Glance article, Emma van Grinsven and Anna Akhmanova provide an overview of the diverse microtubule arrays present in differentiated animal cells and discuss how these arrays form and function.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 7 March 2025 (decision by week commencing 21 April 2025) and 6 June 2025 (decision by week commencing 28 July 2025).
