The vital staining reactions of the normal and ischaemic testis were investigated in 20 rats by the use of varying amounts of trypan blue and Unna's polychrome methylene blue. The experiments showed that it was possible to distinguish both true and reticulo-endothelial interstitial cell types. The implications of this subdivision are discussed and their importance in relation to neoplasms of the Leydig cells stressed. The distribution of mast cells in the testis is described.

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