The black material seen in paraffin preparations of tissues fixed with osmium tetroxide is not merely reduced osmium. The lipid is still present in combination with osmium. Osmicated lipid globules are insoluble in chloroform or benzene. They regain their solubility in lipid solvents when the blackness caused by osmium tetroxide is bleached away.
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JOURNAL ARTICLE| 01 September 1958
Experiments on the Fixation of Lipids by Osmium Tetroxide
J.T. Y. CHOU
Cytological Laboratory, Department of Zoology, University Museum, Oxford
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1958 The Company of Biologists Ltd.
J Cell Sci (1958) s3-99 (47): 285–288.
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J.T. Y. CHOU; Experiments on the Fixation of Lipids by Osmium Tetroxide. J Cell Sci 1 September 1958; s3-99 (47): 285–288. doi: https://doi.org/10.1242/jcs.s3-99.47.285
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